Picture from von Zelewsky T et al. (2000) Development "The C. elegans Mi-2 chromatin-remodelling proteins function in vulval cell fate ...."

Figure 2. Localization of let-418 and chd-3 mRNAs. Embryos were examined by in situ hybridization. Positive staining is indicated by the dark color. (A-C) embryos were hybridized with a let-418 antisense probe. (A) let-418 maternal RNA is detected in 2-cell embryos. (B) In 28-cell embryos, hybridization appears to be uniform in all cells. (E-G) embryos were hybridized with antisense probe to chd-3. (E) No RNA is detected in 2-cell embryos. (F) 28-cell embryo showing hybridization in all somatic cells. (D,H) No hybridization was observed in embryos hybridized with a let-418 sense probe (compare D and C) and chd-3 sense probe (compare H and G). C. elegans embryos are approximately 45 µm in length.

Picture from von Zelewsky T et al. (2000) Development "The C. elegans Mi-2 chromatin-remodelling proteins function in vulval cell fate ...."

Figure 4. LET-418::GFP reporter expression. (A) 28-cell embryo showing GFP expression in all somatic blastomeres. (B) Nomarski image of the same embryo as in A. (C) GFP is expressed in the nuclei of P(3-8).p cells (arrows, from left to right) of L3 animals. The adjacent and overlying stronger signals correspond to ventral nerve cord nuclei. Anterior is left, posterior right. Scale bar, 10 µm.

Picture from von Zelewsky T et al. (2000) Development "The C. elegans Mi-2 chromatin-remodelling proteins function in vulval cell fate ...."

Localization of chd-3 mRNAs. Embryos were examined by in situ hybridization. Positive staining is indicated by the dark color. (E-G) embryos were hybridized with antisense probe to chd-3. (E) No RNA is detected in 2-cell embryos. (F) 28-cell embryo showing hybridization in all somatic cells. (H) No hybridization was observed in embryos hybridized with a chd-3 sense probe (compare H and G). C. elegans embryos are approximately 45 um in length.

Picture from von Zelewsky T et al. (2000) Development "The C. elegans Mi-2 chromatin-remodelling proteins function in vulval cell fate ...."

Localization of let-418 mRNAs. Embryos were examined by in situ hybridization. Positive staining is indicated by the dark color. (A-C) embryos were hybridized with a let-418 antisense probe. (A) let-418 maternal RNA is detected in 2-cell embryos. (B) In 28-cell embryos, hybridization appears to be uniform in all cells. (D) No hybridization was observed in embryos hybridized with a let-418 sense probe (compare D and C). C. elegans embryos are approximately 45 um in length.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of LET-19 in the T-cell division. Expression of let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Figure 5. Symmetric expression of LET-19 and DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) and let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Lockhead D et al. (2016) Mol Biol Cell "The tubulin repertoire of C. elegans sensory neurons and its context-dependent ...."

(B) Coexpression of alpha-tubulin and beta-tubulin TagRFP-T protein fusions and uIs31 TRN::GFP transcriptional fusion. Left, uIs31(mec-17p::gfp) showing PLM. Center, TagRFP-T tubulin fusion protein. Right, composite image. TagRFP-T was false colored as magenta for enhanced visibility. Expression of isoforms inTRNs is seen as white in the composite overlap of uIs31 marker and TagRFP-T::Tubulin. Images are oriented so that the anterior of the animal is on the left and the ventral side is on the bottom. Scale bar, 50 um.

Picture from Herman MA (2001) Development "C. elegans POP-1/TCF functions in a canonical Wnt pathway that controls cell ...."

POP-1 is asymmetrically distributed at the T cell division. POP-1 expression in T.a and T.p. Whole-mount larvae 5-7 hours post-hatching, were stained with monoclonal anti-POP-1 antibodies and MH27 monoclonal antibodies, which outline hypodermal cells. The V cell (V) and T cell (T) daughters are indicated. (A) In wild-type animals, the level of POP-1 is higher in Vn.a than in the Vn.p, and also higher in T.a than in T.p. Scale bar: 10 um.