Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Fig 3. UNC-44 acts upstream of UNC-33. Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)). (C) Overexpression of UNC-33(S) suppresses unc-44(lf) phenotypes. Each experiment was performed with N>200 animals at least three times. For transgenic animals the results shown here are generated from at least three independent lines. Data are shown as mean +- SD. Student's t-test, ** p<0.01. Scale bar: 10 um.

Picture from AlHarbi S et al. (2023) MicroPubl Biol "Characterizing a standardized BioPart for PVQ-specific expression in C. ...."

Figure 1. Expression and Characterization of Pnlp-17 in PVQ Neurons of C. elegans:A. Pnlp-17::mScarlet::tbb-2 3'UTR expression is specific to PVQ neurons from extrachromosomal arrays in hermaphrodites (top panel) and males (bottom panel). B. Single-copy Pnlp-17::mScarlet transgenes inserted by MosTI (El Mouridi et al., 2022) are easily visible in hermaphrodites. 20x magnification. Scale bars: 100 µm. C. Characteristics and quantification of the short 300 bp nlp-17 promoter. D. Schematic of standard BioPart expression vectors for PVQ expression. Unmodified gfp or mScarlet vectors can be used to identify PVQ neurons. Standard restriction sites allow cell-specific expression with N-terminal (ApaI) and C-terminal (SmaI) fluorophore fusions or complete fluorophore exchange (BsaI). The vectors are compatible with single-copy insertions (MosTI) and expression from extra-chromosomal arrays. E. The short 300 bp promoter is easily synthesized and has been included as a standard feature in an online tool for optimizing transgene design and synthesis (www.wormbuilder.org/transgenebuilder) (Vargas-Velazquez, El Mouridi, Alkhaldi, and Frøkjær-Jensen C, manuscript in preparation).

Picture from Ellefson ML et al. (2009) Mol Biol Cell "Kinesin-1 and cytoplasmic dynein act sequentially to move the meiotic spindle to ...."

Images of mCherry-histone (top row) and GFP: DHC-1 (bottom row) within a wild-type meiotic embryo are shown from a representative time-lapse sequence. The cortex has been highlighted in each image for clarity. 0 s is the start of rotation. GFP:DHC-1 was faintly localized across the meiotic spindle before spindle shortening (T = -120 s). 45-30 s before the start of spindle rotation, DHC-1:GFP began to accumulate on meiotic spindle poles and remained on meiotic spindle poles after spindle rotation (T = +60 s).

Picture from Madhu Bhoomi J et al. (2019) MicroPubl Biol

Figure 1. Effects of S. marcescens and S. epidermidis on egg laying and brood size in wild-type and dbl-1(nk3) populations. (A) Comparison of mean eggs laid between wild-type (WT) and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. The counts of number of eggs laid were reported only for plates with live animals. Error bars represent SEM. n = 7-10 surviving animals each. (B) Comparison of total brood size between wild-type and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. Total brood sizes of animals that desiccated were censored. Error bars represent SEM. n = 7-10 surviving animals each. Within each genetic background, significant differences between the E. coli OP50-fed control and the pathogen-fed populations are marked with asterisks (*, p< 0.05, **, p< 0.001).

Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

Behaviors of plus ends of astral microtubules at the cell cortex. The green box in the schematic embryo indicates the region at the cortex that was magnified. Images of GFP-tubulin and GFP-EB1 were acquired with 0.5 s and 1 s exposure times, respectively. CR, contractile ring. Times (s) are with respect to the onset of posterior movement of the spindle. The scale bar is 5 um.

Picture from Juanico, Iris Y et al. (2021) MicroPubl Biol

Figure 1. : Position of paternal DNA within meiotic embryos. Hermaphrodites expressing GFP::SPCS-1, which labels the endoplasmic reticulum, were mated with control or rmd-2, 3, 6 triple mutant males expressing MEV-1::mCherry, which labels mitochondria. (A, B) Representative z projections of methanol-fixed meiotic embryos stained with DAPI and anti-GFP antibodies. Blue indicates DAPI-stained DNA. Red indicates mCherry-labeled paternal mitochondria from the sperm. Green indicates maternal endoplasmic reticulum labeled with anti-GFP, which labels GFP::SPCS-1. Arrows indicate paternal DNA. Control and rmd-2,3,6 indicate paternal genotypes. The control embryo shown is in anaphase I. The triple mutant embryo shown is in metaphase I. Bar = 5 um. (C) Diagram indicates how sperm DNA position relative to the future posterior tip was measured. PS indicates the distance from the future posterior tip of the ellipsoid embryo to the sperm DNA. EL indicates embryo length from the future posterior tip to the future anterior tip of the ellipsoid embryo. PS was divided by EL and the mean and SEM for the resulting ratios are shown. n indicates the number of embryos analyzed. Results of Students unpaired t test indicated no significant difference (NS) between control and rmd-2,3,6 (p=0.42).