Picture from Ortiz CO et al. (2006) Genetics "Searching for neuronal left/right asymmetry: genomewide analysis of nematode ...."

Expression pattern of gcy-28 reporter gene fusion. Transgenic animals expressing gfp reporter gene fusions are shown. Images are of representative animals from several independent lines. Most transgenic animals are scored in the late larval and adult stage and contain otIs151 in the background to facilitate the identification of the ASE neurons. (R) gcy-28prom::gfp. VNC, ventral nerve cord; HG, head ganglia; N, non-neuronal cells. The animal is mosaic and does not show muscle expression.

Picture from Vanderzalm PJ et al. (2009) Development "C. elegans CARMIL negatively regulates UNC-73/Trio function during neuronal ...."

(A) Confocal projection of an L1 C. elegans expressing the extrachromosomal array gmEx374 [Pcrml-1::crml-1::gfp]. CRML-1::GFP is expressed in neurons of the head (HG) and tail (TG) ganglia, and in axons that form the nerve ring, the ventral nerve cord (VNC) and the dorsal nerve cord (DNC) (not shown). The position of a CAN is indicated (arrowhead). This CAN is displaced anterior to its normal position, which is indicated by the dashed arrow. A schematic of the animal is presented on the right.

Picture from Spiro Z et al. (2015) PLoS One "Polarity-dependent asymmetric distribution and MEX-5/6-mediated ...."

(D) and in the 2-cell stage (E). The upper images show the YFP signal alone, thelower ones the merge. Scale bar represents 10 microns.1. de Castro E, Sigrist CJ, Gattiker A, Bulliard V, Langendijk-Genevaux PS, Gasteiger E,et al. (2006) ScanProsite: detection of PROSITE signature matches and ProRuleassociatedfunctional and structural residues in proteins. Nucleic acids research 34:W362-365

Picture from Kuno K et al. (2002) J Biol Chem "The Caenorhabditis elegans ADAMTS family gene adt-1 is necessary for ...."

Figure 4. GFP expression pattern of the adt-1 gene in hermaphrodites. A, C, E, and G, GFP expression in transgenic N2 hermaphrodites carrying the adt-1::GFP reporter gene; B,D, and F, Nomarski micrographs of A,C, and E, respectively. A and B, lateral view of the central body region of an adult; C and D, ventral view of the central body region of an adult; E and F, the head region of an adult; G, a young adult hermaphrodite. The fluorescent portions of the vulva, the head ganglion (hg), the ventral nerve cord (vnc), and amphid neurons (an) are indicated. Scale bars = 20 μm.

Picture from Kaitna S et al. (2000) Curr Biol "Incenp and an aurora-like kinase form a complex essential for chromosome ...."

Fig. 5. RNAi of icp-1 causes de-localization of AIR-2. Wild-type and icp-1(RNAi) embryos were fixed and stained with anti-tubulin and anti-AIR-2 antibodies and DNA was stained with 4,6-diamidino-2-phenylindole (DAPI). In wild-type embryos, AIR-2 was detected on (a) meiotic chromosomes, and (b) mitotic chromosomes during metaphase and on the spindle midzone during anaphase. In icp-1(RNAi) embryos at similar stages, discrete localization of AIR-2 is not observed.

Picture from Meister P et al. (2011) Genome Biol "Caenorhabditis elegans Heterochromatin protein 1 (HPL-2) links developmental ...."

(c) Simplified overview of phosphatidyl choline (PC) metabolism in C. elegans. P-choline, phosphorylcholine; CDP-choline, cytidyldiphosphate choline; PC, phosphatidylcholine; PS, phosphatidylserine; PS DC, phosphatidylserine decarboxylase; PE, phosphatidylethanolamine; Ptd-MeEtn, phosphatidylmonomethylethylamine. Intermediates whose levels are altered in hpl-2 mutant worms compared to wild type are shown in red. (e) Metabolism of sphingomyelin and ceramide. De novo sphingomyelin synthesis is mediated by sphingomyelin synthase (SMS), which transfers the phosphorylcholine moiety from phosphatidylcholine (PC) onto ceramide, forming sphingomyelin and diacylglycerol (DAG). In C. elegans there are three SMS genes, sms-1, -2, and -3. Ceramide can be generated either by the action of sphingomyelinase (SMase) or by de novo synthesis through the enzyme ceramide synthase (CerS, lagr-1, hyl-1 and hyl-2 in C. elegans). (f) hpl-2 regulates sms-3 mRNA levels. hpl-2/N2 ratios for sphingomyelin synthase sms genes at different developmental stages. Experiments were performed on three independent synchronized L3 cultures and on two adult and embryonic cultures. Reference genes are pmp-3 and cdc-42. Normalization was performed using the mean of both reference genes. Data are the mean of independent biological replicas.

Picture from Petersen SC et al. (2011) J Neurosci "A transcriptional program promotes remodeling of GABAergic synapses in ...."

Figure 4. irx-1::GFP is expressed in remodeling GABAergic motor neurons. A, IRX-1::GFP is restricted to the nucleus of DD motor neurons in wild-type L4 larvae. In unc-55 mutants, IRX-1::GFP is observed in both DD and VD motor neurons (DD5 and VD10 shown for examples). Similar expression patterns were observed for all DD and VD motor neurons. Scale bar, 5 um. B, Quantification of IRX-1::GFP expression in GABAergic motor neurons of L2 larvae. Note that IRX-1::GFP is de-repressed in unc-55 VD motor neurons. ***p< 0.0001; NS, not significant; Fisher's exact two-tailed test.

Picture from Bando T et al. (2005) J Mol Biol "The homeoproteins MAB-18 and CEH-14 insulate the dauer collagen gene col-43 from ...."

(f) Fusion gene constructs, summary of expression patterns and upstream required regions of col-43::gfp and col-43::rfp are shown. Asterisks indicate animal recovered from stage L2d. DP (dauer cycle-specific promoter) and DE (dauer cycle-specific expression) indicate required upstream regions for expression and up-regulation for col-43, respectively (details in the text). (g) Lateral view of transgenic worms harboring pC3894G in the L2d stage. (h) Lateral view of transgenic worms harboring pC1345G in the adult recovered from L2d stage. The arrow and arrowhead indicate col-43::gfp expression in hypodermal cells and ectopic expressions in posterior intestine. Anterior is to the left. The scale bars represent 100 um.

Picture from Morck C et al. (2010) BMC Dev Biol "C. elegans ten-1 is synthetic lethal with mutations in cytoskeleton regulators, ...."

(A-E) Embryonic expression of ten-1a::gfp. The asterisk in (A) indicates the anterior cluster of GFP-positive cells in a 150 min embryo. Arrowheads in (B) indicate intercalating hypodermal cells, while the asterisk indicates a cluster of pharyngeal precursor cells in a 300 min embryo. (C-E) Shows that pharyngeal cells (p), intestinal (i) and some neurons (n) express ten-1a::gfp in 350 min(dorsal view), 1.5-fold, and 2-fold stage embryos, respectively. (F-G) Show a L1 larva and the head of an adult, respectively. Note expression in the marginal cells (mc1 and mc3), head ganglion neurons (hg), the intestine (i) and the M2 pharyngeal neuron (M2). (H-J) Show a L1 ten-1(ok641) mutant larva expressing ten-1a::gfp. Note the deformed posterior end (indicated by arrowhead in H), and the poor connection between the posterior intestinal cells(indicated by arrowheads in J). (I) is an overlay of (H) and (J). The scale bar in (A) represents 10 um and applies to all panels except (H-J), which have a separate scale bar.

Picture from Nonet ML et al. (1999) Mol Biol Cell "UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and ...."

Figure 4. UNC-11 is enriched in synaptic regions of C. elegans. Confocal images of adult nematodes prepared for immunohistochemistry using both affinity-purified rabbit anti-UNC-11 antisera and affinity-purified mouse anti-synaptotagmin antisera. Animals were double stained for UNC-11 with rhodamine-conjugated secondary antibody and for synaptotagmin with FITC-conjugated secondary antibody: wild-type (A-E), unc-11(e47) (F and G), and unc-11 (n2954) (H and I). Anterior is left in all images. Panel C is the merge of panels A and B. A representative nerve ring, ventral nerve cord (V. N. C.), dorsal nerve cord (D. N. C.), and coelomocyte are labeled in panel D. Insets in panels A-C and H de- pict localization of UNC-11 staining in neuronal cell bodies. Bar, 10 um.