Picture from Li P et al. (2016) Neuron "Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite ...."

Figure S2. Di-leucine motifs determine polarized protein localization (Asso. w/ Figure 2).(A) Schematic diagram of the C. elegans intestine epithelial cells. The top section shows the basolateral(red) membrane, and the middle section shows the apical (blue) and the basolateral (red) membranes.(B-C) Middle section (B) and top section (C) of GFP-tagged SER-1 localization in intestine epithelial cells.White arrows point to the basolateral membrane. Yellow arrows point to the apical membrane. Scale barsare 10um.(D-E) Middle section (D) and top section (E) of GFP-tagged di-leucine motif mutant SER-1 localization inintestine epithelial cells. White arrows point to the basolateral membrane. Yellow arrows point to the apicalmembrane.(F-G) mCD8-GLR-1C-GFP is co-expressed with cytosolic tdTomato in wild-type (F) and unc-101(m1) (G)animals. Arrows point to the axonal region of the RIA neuron. Scale bar is 10um.(H-I) mCD8-SER-1C(DNSLL)-GFP with the dendritic di-leucine motif is co-expressed with the cytosolic tdTomato in wild-type (H) and unc-101(m1) (I) animals. Arrows point to the axonal region of the RIAneuron. Scale bar is 10um.(J-K) GFP-tagged wild type (J) or mutant GLT-4 (K) is co-expressed with the cytosolic tdTomato in RIA.Arrows point to the dendritic region of the RIA neuron. Scale bar is 10um.

Picture from Gaglia MM et al. (2012) PLoS Genet "Genes that act downstream of sensory neurons to influence longevity, dauer ...."

A-D. Expression pattern of GFP-fused MCT-1 protein in mct-1p::mct-1::GFP transgenic animals. Bright field image (A) and green fluorescence image (B) of an L4 mct-1p::mct-1::GFP transgenic larva. Magnified bright field image (C) and green fluorescence image (D) of dotted box regions in A and B respectively. E-H. The expression of GFP-fused MCT-1 protein in the mct-1p::mct-1::GFP transgenic animal did not overlap with the signal from amphid sensory neurons labeled with the Di dye. Bright field image (E), green fluorescence image (F), red fluorescence image (G) and overlay of green and red fluorescence images (H) of the head of an L3 mct-1p::mct-1::GFP transgenic animal. Arrowheads indicate cell bodies of amphid sensory neurons that are stained with Di dye. The arrow indicates body wall muscle that expresses a muscle-specific myo-3p::RFP transgene, used as a marker for the generation of the transgenic animals.

Picture from Gaglia MM et al. (2012) PLoS Genet "Genes that act downstream of sensory neurons to influence longevity, dauer ...."

Figure 7. Overexpression of mct-1 extends lifespan. A-D. Expression pattern of GFP-fused MCT-1 protein in mct-1p::mct-1::GFP transgenic animals. Bright field image (A) and green fluorescence image (B) of an L4 mct-1p::mct-1::GFP transgenic larva. Magnified bright field image (C) and green fluorescence image (D) of dotted box regions in A and B respectively. E-H. The expression of GFP-fused MCT-1 protein in the mct-1p::mct-1::GFP transgenic animal did not overlap with the signal from amphid sensory neurons labeled with the Di dye. Bright field image (E), green fluorescence image (F), red fluorescence image (G) and overlay of green and red fluorescence images (H) of the head of an L3 mct-1p::mct-1::GFP transgenic animal. Arrowheads indicate cell bodies of amphid sensory neurons that are stained with Di dye. The arrow indicates body wall muscle that expresses a muscle-specific myo-3p::RFP transgene, used as a marker for the generation of the transgenic animals. I-J. Expression pattern of GFP-fused MCT-1 protein in daf-10(m79) mutants, including representative green fluorescence and bright field images (I) and quantification of fluorescence intensity (J). Error bars represent s.e.m. (* p,0.05, Student's t-test). K. Overexpression of mct-1::GFP lengthens lifespan. Three independent lines of mct-1 transgenic animals lived slightly but significantly longer than control animals. Statistical analysis of the lifespan data presented in this figure and additional repeats is included in Table S1B.

Picture from Nikonorova IA et al. (2022) Curr Biol "Isolation, profiling, and tracking of extracellular vesicle cargo in ...."

Figure 3. Nucleic acid binding transporter SID-2 is specifically enriched in the male- specific ciliated EV-releasing neurons (EVNs) and is shed in the form of EVs to the environment(A) Validation protocol for putative ciliary EV cargo. (B and C) EV release of SID-2::mScarlet and PKD- 2::GFP from cilia of CEM neuron of the head (B) and RnB neuron of the tail (C). (C) shows ray #7 in the process of shedding SID-2::mScarlet as evidenced by continuous fluorescence signal spanning the ciliary base, ciliary shaft, and the outside cloud of EVs. Other clouds on (C) are not labeled as EV release events because it was not clear from which cilium they originated. Resolution of the imaging technique was 140 nm in lateral di- rection and 400 nm in axial direction; thus, smaller EVs appear as clouds rather than individual EVs. Areas of intense inherent autofluorescence of the male tail are shaded with gray and labeled as au- toflu. Images show 3D renderings: on merged im- ages green and magenta colors represent different reporters, on images showing individual channels colors code for Z-depth-3.5 mm for (B) and 6.5 mm for (C).Ci, cilia; CEM, cephalic male neuron; HOB, hook neuron type B; RnB, ray neuron type B. Black and white maximum intensity projections of (B) and (C) and other data related to SID- 2::mScarlet expression are included in Figure S3.