Figure 1. The
mir-57 gene is expressed in posterior sublineages in the posterior regions of the animal. (A) A 3-D projection of a confocal z-stack of a comma stage embryo with a stably integrated mCherry reporter driven by a
mir-57 promoter segment (
pmir-57::HIS-24::mCherry). The
mir-57 reporter (red, or yellow when co-localized with green) is detected only in the posterior cells of the embryo. The embryo is shown in a ventral view with anterior to the left. The embryo is also ubiquitously labeled with GFP for lineaging. (B) A space filling model of the same embryo with expressing cells color coded by their lineage origins (see panel F for color code). Non-expressing cells are gray and rendered partially transparent. Note the
mir-57 expressing cells are clustered within the posterior region of embryo. (C) In situ hybridization of a one-and-a half fold wild type (N2) embryo using a DIG labeled anti-sense LNA
mir-57 probe (See Materials and Methods). The anti-DIG staining is apparent as darker regions over the tail (arrowhead). (D) The same in situ staining for
mir-57(
gk175) deletion embryos. No apparent staining was seen in the tail region (arrowhead). (E) A 3-D projection of an L1 larva expressing both
mir-57 (red) and the lineaging marker (green). Most expressing cells are seen in the tail of the animal except for a few intestinal cells. (F) Embryonic expression of
mir-57 within cell lineages. Expressing cells were detected in the four AB sublineages and the C sublineages beginning from the 200 cell stage. The fates of the expressing cells are color coded below the leaves. The color coding of bars under each sublineage correspond to those used in the 3-D space filling model in (B). By convention, anterior daughters are placed on the left branches. Cell deaths are indicated with X.