SNG-1::GFP is localized to synaptic regions in C. elegans. (A and B) Whole mount immunocytochemistry of adult wild-type animals stained with a primary antibody directed against SNG-1 and a fluorescein isothiocyanate (FITC)-conjugated secondary antibody. (A) Lateral view of the head illustrates the synaptic varicosities along an SAB motor neuron axon (arrowheads). The nerve ring (arrow) of the animal is also visible. (B) Dorsal view shows the dorsal nerve cord (arrow) and small fluorescence puncta (arrowheads) in dorsal sublateral nerve cords.
Figure 1. SNG-1::GFP is localized to synaptic regions in C. elegans. (A and B) Whole mount immunocytochemistry of adult wild-type animals stained with a primary antibody directed against SNG-1 and a fluorescein isothiocyanate (FITC)-conjugated secondary antibody. (A) Lateral view of the head illustrates the synaptic varicosities along an SAB motor neuron axon (arrowheads). The nerve ring (arrow) of the animal is also visible. (B) Dorsal view shows the dorsal nerve cord (arrow) and small fluorescence puncta (arrowheads) in dorsal sublateral nerve cords. (C-F) Whole mount immunocytochemistry of adult jsIs219 animals visualized by incubation with primary antibodies directed against SNT-1 (C and D) and GFP (E and F). Cy3- and FITC-conjugated secondary antibodies are used respectively. (G and H) Merged images. (C, E, and G) Lateral views of the nerve ring and the presynaptic varicosities of SAB neurons. (D, F, and H) Dorsal views of the dorsal and dorsal sublateral nerve cords ([C-H] genotype: jsIs219). Bar, 10 um.
(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.
Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.
(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).