Fig 3. RAB-6.1 is broadly expressed in multiple tissues. Fluorescence from Prab-6.1::GFP in (A) body wall muscles, (B) intestinal epithelia, (C) the gonad, (D) the distal tip cells, and (E) the vulva. Fluorescence from (F) Prab-6.1::GFP and (F') Pglr-1::mRFP, with (E'') images merged. The neuron cell bodies for RMDV, AVA, and AVB are outlined. NR indicated the nerve ring bundle of neurites. Bar, 5 um.
Figure 10. RAB-6.2 and LIN-10 colocalize with RME-8 in neurons. (A-D) Fluorescence from neuron cell bodies (A and C) and ventral cord dendrites (B and D) is shown. (A-B'') Cerulean::RAB-6.2 (A and B) colocalizes with Venus::RME-8 (A' and B'; A'' and B'' show merged). (C-D'') LIN-10::CFP (C and D) colocalizes with Venus::RME-8 (C' and D'; C'' and D'' show merged). Arrowheads indicate colabeled puncta. Bars, 5 um.
Figure 2. RAB-6.2 is broadly expressed in multiple tissues. (A-I) Fluorescence from Prab-6.2::GFP in body wall muscles (A), pharyngeal muscle and head neurons (B), hypodermal cells (C), the seam cells (D), intestinal epithelia (E), coelomocytes (F), vulval epithelia (G), the distal tip cells (H), and the vulval muscles and ventral cord dendrites (I). (J-J'') Fluorescence from Prab-6.2::GFP (J) and Pglr-1::mRFP (J') with images merged (J''). Coexpressing neurons are outlined. Bars, 5 um.
Figure 5. RAB-6.2 associates with Golgi and GLR-1. (A and A') Cerulean:: RAB-6.2 (A) and MANS::YFP (A') in PVC cell bodies. (A'') Merged image showing Cerulean::RAB-6.2 colocalization to puncta with MANS::YFP (arrowheads). (B-C'') GLR-1::CFP (B and C) and Venus::RAB-6.2 (B' and C') in PVC cell bodies (B-B'') and dendrites (C-C''). (B'' and C'') Merged images show colocalization at specific points (arrowheads). (D-F) Subcellular localization of the indicated GFP::RAB-6.2 variant: wild type (D), GDP-locked mutant (E), and GTP-locked mutant (F). Bars, 5 um.
(A, A'-D, D') Expression of the frizzled homolog T23D8.1 monitored using GFP. PT23D8.1::GFP is expressed in a few cells of the nerve ring (A, A'). Further, it is expressed in the intestine (B, B'), one cell on the ventral side of the terminal bulb of the pharynx (arrow in C, C') and two cells around the anus (D, D').