Picture from Zhang Y et al. (2012) Curr Biol "C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on ...."

Figure 1. nrf-5 Is Required for Cell Corpse Clearance (A) Time-course analysis of cell corpses during embryonic development was performed in wild-type (N2, open bar), nrf-5(qx16) (black bar), or nrf-5(sa513) (gray bar). At least 15 embryos were scored at every stage. Data are shown as mean 6 SEM. Data derived from WT and nrf-5(qx16) or WT and nrf-5(sa513) were compared by unpaired t test. p < 0.0001; all other points had p > 0.05. (B) Embryonic cell deaths were followed in WT (N2, n = 3) and nrf-5(qx16) (n = 3) embryos. The average number of total cell deaths (6SEM) is shown in parenthesis. (C) The duration of 33 corpses from WT (N2, open bar, n = 3) and 61 corpses from nrf-5(qx16) (black bar, n = 4) was monitored. The average duration (6SEM) is in parenthesis. (D) nrf-5(sa513) does not enhance the engulfment defect in ttr-52 and ced-7 mutants. Cell corpses were scored in the head region of 4-fold embryos. At least 15 animals were scored in each strain. Data are shown as mean 6 SEM. The number of cell corpses in WT was compared with that in ttr-52(tm2078) or nrf- 5(sa513). For other points, single ced mutants were compared with ced;nrf-5 double mutants, and ttr-52;nrf-5 double mutants were compared with nrf-5 single mutants. p < 0.0001; all other points had p > 0.05. (E) Schematic diagram of the cell corpse engulfment pathway.

Picture from Zhang Y et al. (2012) Curr Biol "C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on ...."

Figure S2. nrf-5 Is Expressed in Body Wall Muscle Cells, Related to Figure 2 (A-D''') DIC and fluorescent images of wild-type embryos (A-A''', D-D''') or larvae (B-C''') transgenic for Pnrf-5GFP and Pges-1mCHERRRY (A-C''') or Pttr-52mCHERRY (D-D'''). The GFP expression driven by the nrf-5 promoter is distinct from that of mCHERRY controlled by the ges-1 and ttr-52 promoters which drive gene expression specifically in intestine cells. (E-F') DIC and fluorescent images of wild-type animals expressing ssGFP::mNRF-5 driven by the nrf-5 promoter. Diffuse GFP signal was observed throughout the worm. GFP labeling was also found on the basal surface of pharyngeal cells (E, E', arrowheads) and basal and lateral membranes of the intestine (F, F', arrowheads). (G-H''') DIC and fluorescent images of wild-type animals transgenic for both Pnrf-5GFP::mNRF-5 and Pmyo-3mCHERRY (G-G''') or both Pnrf-5GFP::mNRF-5 and Pvha-6mCHERRY (G-G'''). Non-secretory GFP::mNRF-5 was seen in body wall muscle cells, overlapping with Pmyo-3mCHERRY, but not in intestine cells, which are marked by Pvha-6mCHERRY, except for some GFP signal in gut granules (arrowheads). Scale bars in all panels represent 5 um.

Picture from Zhang Y et al. (2012) Curr Biol "C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on ...."

(G-H''') DIC and fluorescent images of WT animals transgenic for both Pnrf-5GFP and Pmyo-3 mCHERRY. Scale bars in all panels represent 5 um.

Picture from Zhang Y et al. (2012) Curr Biol "C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on ...."

Figure 2. NRF-5 Is a Secretory Protein from Body Wall Muscle Cells and Clusters around Apoptotic Cells (A-F' ) Differential interference contrast (DIC) and fluorescent images of WT (A-B', D-E') or ced-3(n717) embryos (C, C', F, F ' ) that carry PhspNRF-5::mCHERRY (A, A', C, C' ), PhspNRF- 5(21-551)::mCHERRY (B, B' ), Pced-1ssGFP:: mNRF-5 (D, D', F, F'), or Pced-1GFP::mNRF-5 (E, E'). Arrows indicate apoptotic cells, and arrowheads point to accumulated fluorescent proteins in the extraembryonic cavity. Cell corpses indicated by yellow arrows are shown at 33 magnification in the insets. (G-H''') DIC and fluorescent images of WT animals transgenic for both Pnrf-5GFP and Pmyo-3 mCHERRY. Scale bars in all panels represent 5 um.

Picture from Loer CM et al. (2015) Genetics "Cuticle integrity and biogenic amine synthesis in Caenorhabditis elegans ...."

(B) ptps-1-GFP reporters. Left: head showing NSM expression plus other neurons and nonneuronal cells, including anterior intestine (dorsal-ventral view, maximum intensity projection) (MIP); strain OH11619, described by Zhang et al. (2014). Right: vulval region showing expression in neurons VC4, 5 (weak) and HSN, plus lateral seam cells (lateral view, MIP); strain CZ18321.

Picture from Zhang H et al. (2001) Development "The novel C. elegans gene sop-3 modulates Wnt signaling to regulate Hox gene ...."

Figure 4. sop-3::gfp reporter genes are expressed in many nuclei throughout development. (A) The structures of sop-3::gfp reporter genes. (B,C) Expression in comma stage embryo (B, Nomarski image; C, fluorescence image). (D) Expression in late L3 male. (E) Expression in ray precursor cells and many additional cells of an L3 male tail. (F) Expression in proliferating vulval cells of an L3 hermaphrodite. Large irregular fluorescence particles are gut autofluorescence.

Picture from Zhang L et al. (2007) Mol Cell "Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA ...."

(C) Fluorescence images showing the expression of a functional AIN-2::GFP from a single-copy-integrated transgene. The GFP signal was visible in all tissues examined.

Picture from Zhang L et al. (2007) Mol Cell "Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA ...."

Figure S1. ain-2 encodes an ubiquitously expressed homolog of AIN-1. (A) Schematic illustration of the structure of ain-2 (B0041.2) and the tm1863 mutation. Only one of the three predicted potential alternative splicing forms is shown (wormbase.org). The tm1863 mutation, which lacks the region indicated, resulted in a truncated protein with a new in-frame ATG at the position indicated. (B) Aligned black regions indicate homologous sequences in AIN-1 and AIN-2. (C) Fluorescence images showing the expression of a functional AIN-2::GFP from a single-copy-integrated transgene. The GFP signal was visible in all tissues examined.

Picture from Ho J et al. (2018) MicroPubl Biol "Characterizing Dyneins Role in P-cell Nuclear Migration using an Auxin-Induced ...."

Figure 1. Auxin-induced degradation of dynein causes a P-cell nuclear migration defect. Average number of GABA neurons in L4 animals that express the TIR1 gene (an auxin-induced E3 ubiquitin ligase; Zhang et al., 2015) specifically in P cells. Animals either expressed the DHC-1 degron tag (UD551) or did not express the DHC-1 degron tag (UD550). These animals (UD551 and UD550) were either exposed to auxin (+) or not (-) as L1 animals. Statistical significance calculated by t-test with p-value<0.0001. The mean is marked with error bars denoting 95% CI.

Picture from Barriere A et al. (2012) PLoS Genet "Coevolution within and between regulatory loci can preserve promoter function ...."

Figure 1. The expression pattern of unc-47 is conserved despite divergent regulation. (A) Both C. elegans and C. briggsae promoters fused upstream of GFP in their endogenous trans-regulatory environments drive expression in all 26 GABAergic neurons (green). However, the C. briggsae promoter placed in the C. elegans trans environment additionally drives expression in SDQR and SDQL (blue). (B) Those cells were identified as SDQR/L based on their position and their characteristic projections. (C) For each combination of promoter and trans-regulatory environment, expression in SDQR and SDQL is presented. C. elegans is represented by straight lines, C. briggsae by wavy lines. Frequency of expression is represented by the width, and intensity of expression relative to D-type neurons by the height of black boxes. Number of individuals expressing and total number of individuals scored is indicated underneath. Measurements for independent strains are given in Figure S1. Differences in frequency of expression in SDQR are significant for all comparisons: C. elegans and C. briggsae promoters in C. elegans trans environment (p = 8.1610212), C. elegans and C. briggsae promoters in C. briggsae trans environment (p = 1.561028), C. elegans promoter in C. elegans and C. briggsae trans environments (p = 4.8610211), C. briggsae promoter in C. elegans and C. briggsae trans environments (p = 2.4610214). (D) Interpretation of the results presented in panel C. Both C. elegans and C. briggsae promoters in their endogenous trans environments drive low levels of expression in SDQR and SDQL, while disruption of the endogenous interactions either drives high levels of expression (C. briggsae promoter in C. elegans) or abolishes expression (C. elegans promoter in C. briggsae).