Picture from Zhang Q et al. (2018) Cell "The Mitochondrial Unfolded Protein Response Is Mediated ...."

(G) Representative of three center images of confocal photomicrographs of animals expressing egl-20p::mcherry or egl-20p::egl-20p::mCherry in combinationwith rgef-1p::Q40::yfp. Scale bar represents 50 um.

Picture from Zhang Q et al. (2018) Cell "The Mitochondrial Unfolded Protein Response Is Mediated ...."

Figure S7. EGL-20 Is Expressed in Neurons in C. elegans, Related to Figure 7(A) Representative photomicrographs of animals expressing neuronal EGL-20; hsp-6p::gfp in WT, ser-4(ok152), ser-7(tm1325), ser-1(ok345), or ser-1(ok345); ser7(tm1325) double mutants background.(B) Representative photomicrographs of animals expressing neuronal EGL-20; dve-1p::dve-1::gfp in WT or mod-1(ok103) background.(C) Representative photomicrographs of egl-20p::egl-20::mcherry in WT, vps-35(ok1880), or mig-14(ga62) background.(D) Quantitative PCR of egl-20 mRNA level. Synchronized Day 1 adult animals of WT, rgef-1p::Q40::yfp were collected for qPCR. ns denotes p > 0.05 via t test.Error bar indicates the SEM from six biological replicates.(E) Immunoblots of mCherry and YFP expression in egl-20p::egl-20::mcherry animals in the presence or absence of neuronal Q40. Anti-tubulin serves as a loadingcontrol.(F) Quantitative PCR of Wnt target genes mRNA level. Synchronized Day 1 adult animals of WT and rgef-1p::Q40::yfp were collected for qPCR. *p < 0.05,**p < 0.01, ns denotes p > 0.05 via t test. Error bars indicates the SEM from six biological replicates.(G) Representative of three center images of confocal photomicrographs of animals expressing egl-20p::mcherry or egl-20p::egl-20p::mCherry in combinationwith rgef-1p::Q40::yfp. Scale bar represents 50 um.(H) Representative photomicrographs of animals expressing egl-20p::egl-20p::mCherry in combination with rgef-1p::yfp or rgef-1p::Q40::yfp. Scale bar represents 50 um.

Picture from Zhang S et al. (2004) Cell "Combinatorial marking of cells and organelles with reconstituted fluorescent ...."

(A) Punc-24gfp is expressed in many adult cells. (B) Punc-24nzgfp and Pmec-2czgfp are coexpressed only in six touch receptor neurons.

Picture from Zhang S et al. (2004) Cell "Combinatorial marking of cells and organelles with reconstituted fluorescent ...."

Figure 4. Use of recGFP to Identify Cells Coexpressing Two Genes. (A) Punc-24gfp is expressed in many adult cells. (B) Punc-24nzgfp and Pmec-2czgfp are coexpressed only in six touch receptor neurons. (C) Pmec-3nzgfp and Pegl-44czgfp are coexpressed only in the two FLP neurons.

Picture from Zhang Y et al. (2009) Cell "SEPA-1 mediates the specific recognition and degradation of P granule ...."

(A and B) No obvious expression of sepa-1 could be detected by anti-SEPA-1 antibody in embryos before the 16-cell stage. An 8-cell-stage embryo is shown. (A) DAPI image of the embryo shown in (B).(C and D) The number of SEPA-1 aggregates increases during early stages of embryogenesis (from 20-cell to 100-cell stages). (C) DAPI image of the embryo shown in (D).(E and F) SEPA-1 aggregates are almost undetectable in a comma-stage embryo. (E) Nomarski image of the embryo shown in (F).(G-J) SEPA-1 is not present in germline P granules. P granules are detected by anti-PGL-3 antibody. (G)-(J) are confocal images.

Picture from Zhang F et al. (2011) Genetics "A left/right asymmetric neuronal differentiation program is controlled by the ...."

ztf-5 transl::GFP expression pattern in midembryonic stages. Embryos are contained within the gonad of the mother.

Picture from Zhang F et al. (2011) Genetics "A left/right asymmetric neuronal differentiation program is controlled by the ...."

Figure 3 lsy-27 is expressed and acts during the initiation but not during the maintenance phase of left/right asymmetry control. (A) Expression pattern of lsy-27fosmid::yfp (shown in Figure 2A) at different embryonic stages. The embryos appear slightly deformed as they are squished together in the gonad of an adult animal. The white arrow indicates the ASEL neuron shortly after birth based on colocalization with a bilateral ASE-specific reporter otIs232(che-1::mCherry) (not shown). The lsy- 27transl::gfp (shown in Figure 2A) shows a similar expression pattern except that, due to its failure to be expressed in the germline, we see only the onset of expression when zygotic gene expression starts in the early embryo. Bar, 10 mm.

Picture from Zhang Y et al. (2009) Cell "SEPA-1 mediates the specific recognition and degradation of P granule ...."

(K and L) Expression of sepa-1::gfp is first detected in 16-cell-stage embryos. SEPA-1 distribution is mainly diffuse, with a few aggregates. (K) Nomarski image of the embryo shown in (L).(M-P) The number of SEPA-1::GFP aggregates increases during early stages of embryogenesis (before 100-cell stage) and decreases as development proceeds. SEPA-1::GFP is still diffusely distributed in a comma-stage embryo (O and P) but is almost undetectable in a 4-fold-stage embryo (data not shown). (M and O) Nomarski images of the embryos shown in (N) and (P), respectively.(Q and R) SEPA-1::GFP is localized in the intestine at larval stages, especially in the anterior and posterior pairs of intestine cells. (R, upper panel) Nomarski image.

Picture from Zhang Y et al. (2010) Genetics "Mutations in Caenorhabditis elegans eIF2beta permit translation initiation ...."

Figure 1. Construction of a GFP reporter for screening translation initiation mutants. (A) Transcriptional reporter of act-1::GFP containing the native ATG translation start codon of the GFP gene. (B) GFP expression from act-1::GFP reporter in wild-type worms. (C) Non-AUG GFP reporter carrying a mutation that alters the ATG translation start codon to GTG. (D) GFP expression from GTG-GFP reporter in wild-type worms. Reporter expressions shown in both B and D were obtained from extrachromosomal arrays.

Picture from Zhang F et al. (2011) Genetics "A left/right asymmetric neuronal differentiation program is controlled by the ...."

Expression pattern of the lsy-27 paralog ztf-25. A: Gene and allele structure of ztf-25. A translational reporter was generated by fusing the genomic DNA containing 1.3 kb upstream of ATG to GFP and a heterologous 3'UTR. Note the proximity of ztf-25 and its closest paralog, lsy-27. B: ztf-5 transl::GFP expression pattern in midembryonic stages. Embryos are contained within the gonad of the mother.