Figure 1.
bar-1(
ga80) suppresses
pry-1(
gk3682) phenotype involving
vit-2 transcription, lipid levels, vulval development, and lifespan:(A) qRT-PCR analysis of
vit-2 in
day-1 adults of
pry-1(
gk3682),
bar-1(
ga80), and
pry-1(
gk3682);
bar-1(
ga80) animals. The relative normalized expression has been plotted. (B) Quantification of Oil Red O signal in mutant worms. (C) Muv phenotype in adults. (D) Lifespan analysis of animals. (E) A model of
pry-1 and
bar-1 function based on results presented in this study. In addition to
bar-1,
vit-2 expression may be regulated by other factors. Our genetic analysis suggests that the
pry-1-
bar-1 pathway regulates lipid levels via both
vit-2-dependent and -independent manners. Histograms in A, B, and C show the means of at least three replicates and the error bars represent the standard error of the mean (SEM). The lifespan data in D is based on three independent batches. The p values are represented by stars (**** p<0.0001). The mean lifespan of N2 was 15.1±0.3 days,
pry-1(
gk3682) 3.1±0.9 days,
bar-1(
ga80) 10±0.2 days, and
pry-1(
gk3682);
bar-1(
ga80) 8.9±0.3 days. p <0.0001 for
pry-1 vs. N2, p<0.0001 for
bar-1 vs. N2, p<0.0001 for
pry-1;
bar-1 double vs.
pry-1, and p = 0.02 for
pry-1;
bar-1 double vs.
bar-1