(C-H) Expression of med-1::GFP::MED-1. (C and D) 8-cell stage; (E and F) 16-cell stage; (G and H) 28-cell stage. Anterior is to the left, and dorsal is up.
Expression of C41C4.8 in C. elegans. The upstream portion of C06A1.1 encompassing regulatory region was cloned into plasmid pPD95.77. Resulting plasmids were microinjected and transgenic lines were obtained as described in Section 2. (E-H) C06A1.1. (E, F) Four- to five-day-old transgenic C. elegans. (G, H) embryos. (E, H) Differential interference contrast micrographs. (F, G) Fluorescence micrographs.
(F-H) Staining of WT animals with SYD-9 antibody N (F and G) and SYD-9 antibody C (H). SYD-9 is expressed in the nuclei of body wall muscles (arrow) and ventral cord motoneurons (arrowhead). At higher magnification, SYD-9 expression shows a distinct subnuclear pattern similar to that of SYD-9::GFP; in particular, antibody C shows a speckle-like pattern (G and H). Antibody N staining in the retrovesicular ganglion neurons (G) and antibody C staining in the nerve ring ganglion neurons (H) are shown. (I and J) Western blot analysis of mixed-stage C. elegans lysates by using antibody N (I) and antibody C (J). Tubulin is the loading control for lysate. (Scale bars: A, 20 um; B and F, 3 um; C-E, G, and H, 1 um.)
(A) and (C): DIC images of the embryos shown in (B) and (D). E-H. At postembryonic stages, epg-11::gfp is expressed in pharyngeal cells (E,F, arrows) and vulval cells (G,H, arrow). (E) and (G): DIC images of the animals shown in (F) and (H).
Fig. 2. Expression of C41C4.8 and C06A1.1 in C. elegans. The upstream portion of C41C4.8 and C06A1.1 genes encompassing regulatory region was cloned into plasmid pPD95.77. Resulting plasmids were microinjected and transgenic lines were obtained as described in Section 2. (A-D) C41C4.8, (E-H) C06A1.1. (A, B, E, and F) Four- to five-day-old transgenic C. elegans. (C, D, G, and H) embryos. (A, D, E, and H) Differential interference contrast micrographs. (B, C, F, and G) Fluorescence micrographs.
Tissue expression pattern of C. elegans kqt-2. C, expression of kqt-2::GFP exclusively in intestinal cells. H, low magnification views of kqt-2::GFP expression throughout the entire worm. GFP constructs were COOH-terminal translational fusions from genomic subclones (see Experimental Procedures). Scale bars: 20 μm (C) and 100 μm (H).
Figure 4. psa-1 and psa-4 Are Expressed Ubiquitously. Expression of psa-1::GFP (A-D) and psa-4::GFP (E-H). Anterior is to the left and ventral is to the bottom. (A and E) Whole L1 animals examined by epifluorescent microscopy. (B-D) and (F-H) Confocal images of the tail region in L1 animals. (C) and (G) are Nomarski images of the same regions as (D) and (H), respectively. The T cells are in telophase in (C-D) and (G-H).
Expression pattern of C. elegans kcc-3. F-H, GFP fluorescence in animals carrying a kcc-3 promoter::gfp::kcc-3 3' UTR reporter transgene. kcc-3 is expressed in glia in G, the head and H, tail.
(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.