Picture from Zallen JA et al. (1998) Cell "The conserved immunoglobulin superfamily member SAX-3/Robo directs multiple ...."

Figure 4. Expression of sax-3::GFP. (A) Comma stage embryo. The anterior cells that express sax-3::GFP include developing neurons and epidermal cells. The ventral cells in the midbody are the ventral motor neuron precursors. Much weaker expression is observed in the lateral epidermal cells.(B) L1 stage larva. Expression is high in head motor neurons and interneurons (arrow). Muscles of the head and ventral body also express the transgene (arrowheads). Epidermal expression is absent by this time. No expression is observed in the immature HSN motor neurons.(C) L2 stage larva. The HSN motor neuron is extending a growth cone toward the ventral midline and expresses sax-3::GFP at high levels. Arrowhead denotes muscle expression.(D) L3 stage larva. The HSN axon has reached the ventral midline, but is not yet extending along the longitudinal bundles (arrow). The ventral muscles also express sax-3::GFP (arrowhead).(C) is a confocal image obtained by Tim Yu; all other images are taken using conventional fluorescence microscopy. Anterior is at left and dorsal is up in all panels. Scale bars = 10 μm.

Picture from Ortiz CO et al. (2006) Genetics "Searching for neuronal left/right asymmetry: genomewide analysis of nematode ...."

Figure 5. Expression patterns of gcy reporter gene fusions. Transgenic animals expressing gfp reporter gene fusions are shown. Images are of representative animals from several independent lines (see Table 1 for a list of transgenic reporter arrays used). Most transgenic animals are scored in the late larval and adult stage and contain otIs151 in the background to facilitate the identification of the ASE neurons (see materials and methods); blue circles indicate ASER; red circles indicate ASEL. The quantification of the left/right asymmetric expression in ASE is shown in Figure 6. (A) gcy-1prom::gfp. Dorsal view (left) and ventral view (right) of two different focal plains of the head region. The inset in the right panel shows the overlap of the gfp signal with otIs133, an AIY-expressed rfp marker (lateral view). (B) gcy-3prom::gfp. Dorsal view of the head region (left) of an animal whose amphid sensory neurons have been filled with DiI. (Right) A full-length worm with expression in the PVT interneuron. (C) gcy-4prom::gfp. Dorsal view of the head region. (D) gcy-14prom::gfp. Dorsal view of the head region. (E) gcy-20prom::gfp. Dorsal view of the head region (left). A full-length worm with expression in the excretory system (right). EXG, excretory gland cell; EXC, excretory canal cell. (F) gcy-7prom::gfp. Lateral view. Expression in ASEL, but not expression in the excretory canal cell (EXC), has been previously reported (Yu et al. 1997). (G) gcy-2prom::gfp. Lateral view of the head region. A defined subset of the amphid neurons are filled with DiI to allow for easier assessment of cell position. (Inset) A dorsal view illustrating bilateral symmetry of gfp-expressing cells. (H) gcy-11prom::gfp. Lateral view of the head region. The strong neuronal expression (N) in the pharynx is likely due to the injection marker, but pharyngeal muscle expression is due to the reporter gene. (I) gcy-13prom::gfp. Dorsal view of the head region. (Inset) A lateral view of a DiI-filled animal. ( J) gcy-15prom::gfp. Lateral view of the head region. A defined subset of the amphid neurons are filled with DiI to allow for easier assessment of cell position. (Inset) A ventral view to illustrate bilateral symmetry. (K) gcy-17prom::gfp. Dorsal view of the tail region. (Inset) A DiI-filled animal. (L) gcy-18prom::gfp. Lateral view of the head region. (Inset) A lateral view of gfp expression in relation to the rfp expression from otIs133, an AIY-specific cell marker. (M) gcy-21prom::gfp. Lateral view of the head region of a DiI-filled animal. (Inset) A ventral view illustrating bilateral symmetry. (N) gcy-19prom::gfp. Lateral view (left) and dorsal view (right) of the head region. (Inset, left) The overlap of the gfp signal with DiI-filled IL2 neurons. (O) gcy-25prom::gfp. (Left) An oblique view of the head region. (Right) A lateral view of the tail region. (P) gcy-23prom::gfp. Ventral view of the head region. (Q) gcy-27prom::gfp. Dorsal view of the head region. (Inset) A lateral view of a DiI-filled animal. (R) gcy-28prom::gfp. VNC, ventral nerve cord; HG, head ganglia; N, non-neuronal cells. The animal is mosaic and does not show muscle expression. (S) gcy-29prom::gfp. Ventral view of the head region. The reporter is also expressed in AWCL/R and in AVKL/R (not shown in this animal).