Picture from Yi P et al. (2017) Curr Biol "Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C.elegans ...."

(A) Comparison of fluorescence signals in XBX-1 overexpression (OE) and knock-in (KI)lines. Fluorescence in the cell body and dendrite of the KI strain is weaker than that inthe overexpression line. Arrows indicate the cell bodies of PHA/B neurons. Arrowheadsindicate the ciliary bases of phasmid cilia. Scale bar: 5 um.

Picture from Kim KW et al. (2016) BMC Biol "Coordinated inhibition of C/EBP by Tribbles in multiple tissues is essential for ...."

i Fluorescence images of endogenous NIPI-3 expression visualized in the GFP KI strain (fr152). Expression isobserved in the nuclei (yellow arrows) of the epidermis (left panel), the intestine (upper right panel) and the head neurons (lower right panel)

Picture from Kim HS et al. (2014) Genetics "The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell ...."

(A) Wild-type and mig-18(k140) young adults that express MIG-17-Venus.

Picture from Quach TK et al. (2013) PLoS One "Genome-wide microarrray analysis reveals roles for the REF-1 family member ...."

Expression of an integrated transgenic reporter of ftn-1 activity in young adult wild-type animals.

Picture from Kim KW et al. (2018) Neuron "A Neuronal piRNA Pathway Inhibits Axon Regeneration in C.elegans."

Figure 3. prde-1 mRNA Is Present in PLM, and prde-1 Inhibits Axon Regeneration Cell-Autonomously(A) smFISH shows prde-1 mRNAs in the cytoplasm of PLM neuron labeled with Pmec-7-gfp (muIs32). Right bottom shows no probe control.(B) Schematic of gfp KI into prde-1 genomic locus (ju1534). See also Figure S2A.(C) Confocal image of endogenous GFP::PRDE-1 fluorescence in the germline nuclei in the prde-1 (ju1534) gfp KI animals.(D) Schematic of depletion of GFP-tagged PRDE-1 protein using GFP-DEGRON (modeled after Wang et al., 2017).(E) PLM axon regrowth 24 hr post-axotomy. Data are represented as mean +- SEM. Student's t test. Number of animals is shown within columns. Bottom:representative images of PLM neurons 24 hr post-axotomy. Red-orange arrowhead, site of axotomy.(F) PLM axon regrowth 24 hr post-axotomy in transgenic animals expressing prde-1 driven by tissue-specific promoters for mechanosensory neurons (Pmec-4),motor neurons (Punc-25), or muscle (Pmyo-3) in a prde-1(mj207) background. Data are represented as mean +- SEM. One-way ANOVA followed by Tukey'smultiple comparison tests. Number of animals is shown within columns.

Picture from Gruninger TR et al. (2008) PLoS Genet "Sensory perception of food and insulin-like signals influence seizure ...."

(B) Pplc-3:YFP expression pattern in young adult male. The ventral protractors are labeled. Scale bar 6 um.

Picture from Yi P et al. (2017) Curr Biol "Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C.elegans ...."

Figure S1. Improved fluorescence imaging of C. elegans sensory cilia. Related toFigure 1 and 2.(A) Comparison of fluorescence signals in XBX-1 overexpression (OE) and knock-in (KI)lines. Fluorescence in the cell body and dendrite of the KI strain is weaker than that inthe overexpression line. Arrows indicate the cell bodies of PHA/B neurons. Arrowheadsindicate the ciliary bases of phasmid cilia. Scale bar: 5 um.(B-C) Imaging of the endogenous dynein-2 heavy chain (GFP::CHE-3) movement withan EMCCD camera and a 100x objective (red) or a cMOS camera and a 150x objective(green). A pair of phasmid cilia (numbered as 1 and 2) extend from the PHA or PHBdendritic endings and each consists of the ciliary base, middle segment (m.s.) anddistal segments (d.s.). A stack containing 200 time-lapse frames of the middlesegments were projected at maximal intensity into a single plane to show the overalllocation of multiple particles (also see Movie S1). Note with improved imageacquisition (right), two major tracks appear distinguishable, which were confirmed byplot of normalized intensity across the middle segments in (C). The distance betweenthe two tracks is ~300 nm. Scale bar: 5 um.

Picture from Benard C et al. (2001) Development "The C. elegans maternal-effect gene clk-2 is essential for embryonic ...."

Northern and western analyses of clk-2 at all developmental stages (E, embryos; L1-L4, larval stages; A, young adults; glp-4(bn2ts) adults).

Picture from Pispa J et al. (2008) BMC Dev Biol "C. elegans dss-1 is functionally conserved and required for oogenesis and larval ...."

dss-1 mRNA is expressed at different larval ages (L1-L4) and in adult worms (ya, young adult) as shown by RT-PCR. act-4, actin(M03F4.2).

Picture from Carnell L et al. (2005) J Neurosci "The G-protein-coupled serotonin receptor SER-1 regulates egg laying and male ...."

B, C, Expression pattern of Pser-1::gfp in L1 hermaphrodite larvae. Pser-1::gfp(egEx21) contains an 8 kB upstream ser-1 promoter and is expressed in pharyngeal muscle, neurons in the head, ventral cord, and tail of L1 larvae. D, Expression of Pser-1::gfp(egEx21) in young adults. Pser-1::gfp is expressed in the vulval muscles in young adult hermaphrodites.