Picture from Zou W et al. (2009) PLoS Genet "Caenorhabditis elegans myotubularin MTM-1 negatively regulates the ...."

Figure S5. Plasma membrane localization of MTM-1 does not require the activities of PI3-kinases. (A) MTM-1::GFP is expressed in many different cell types. DIC and fluorescence images of wild-type animals expressing Pmtm-1mtm-1::gfp are shown. MTM-1::GFP was seen in distal tip cells (i, ii), coelomocytes (iii, iv) and vulva cells (v, vi). Bars: 10 um. (B) Both full-length and truncated GFP::MTM-1 were stably expressed in C. elegans. Lysates were prepared from 200 adult transgenic worms carrying Pced-1GFP::MTM-1, Pced-1GFP::MTM-1(Delta GRAM), Pced-1GFP::MTM-1(Delta PTP) or Pced-1GFP::MTM-1(Delta CC) and western blot analysis was performed using an anti-GFP antibody. Full-length GFP::MTM-1 (93 Kd) and the three GFP::MTM-1 truncations (Delta CC: 91 Kd, Delta GRAM: 76 Kd, Delta PTP: 65 Kd) were all expressed at the expected size. (C) DIC and fluorescence images of MTM-1::GFP in wild-type (i, ii), vps-34(h797) (iii, iv), piki-1(ok2346) (v, vi) and vps-34(h797);piki-1(ok2346) (vii, viii) embryos are shown. The plasma membrane localization of MTM-1::GFP is not affected in the loss-of-function mutants of PI3-kinases. Bars: 5 um.

Picture from Moore LL et al. (2001) J Cell Biol "HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for ...."

HCP-4 localization in C. elegans embryos. (A) Western blot of wild-type embryo extract (60 ug total protein) probed with anti-HCP-4 antibody (diluted 1:500) shows that a single band of 136 kD is present. All other bands, including the band at 70 kD (*), were independent of the primary antibody. Wild-type embryos were stained with DAPI (B), anti-HCP-3 antibody (C), and anti-HCP-4 antibody (D). Approximately equal staged embryos containing both mitotic (M) and interphase cells are shown. Exposure times were normalized relative to the HCP-3 staining. Arrow indicates prophase nucleus with nuclear staining. Bar, 5 um.

Picture from Kim HS et al. (2014) Genetics "The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell ...."

(A) Wild-type and mig-18(k140) young adults that express MIG-17-Venus.

Picture from Moore LL et al. (2001) J Cell Biol "HCP-4, a CENP-C-like protein in Caenorhabditis elegans, is required for ...."

Figure 2. HCP-4 localization in C. elegans embryos. (A) Western blot of wild-type embryo extract (60 ug total protein) probed with anti-HCP-4 antibody (diluted 1:500) shows that a single band of 136 kD is present. All other bands, including the band at 70 kD (*), were independent of the primary antibody. Wild-type embryos or hcp-4(RNAi) embryos were stained with DAPI (B and E), anti-HCP-3 antibody (C and F), and anti-HCP-4 antibody (D and G). Approximately equal staged embryos containing both mitotic (M) and interphase cells are shown. Exposure times were normalized relative to the HCP-3 staining. Arrow indicates prophase nucleus with nuclear staining. Bar, 5 um.

Picture from Quach TK et al. (2013) PLoS One "Genome-wide microarrray analysis reveals roles for the REF-1 family member ...."

Expression of an integrated transgenic reporter of ftn-1 activity in young adult wild-type animals.

Picture from Gruninger TR et al. (2008) PLoS Genet "Sensory perception of food and insulin-like signals influence seizure ...."

(B) Pplc-3:YFP expression pattern in young adult male. The ventral protractors are labeled. Scale bar 6 um.

Picture from Skipper M et al. (1999) Genetics "Genetic and molecular analysis of fox-1, a numerator element involved in ...."

Figure 4. fox-1 expression analysis. (A) A Western blot with extracts collected from a pure population of L4/adult males (M) and N2 animals at the different stages of development from embryo (E), L1, L2, L3, L4, to young adult (A), was probed with affinity-purified rabbit polyclonal antibody against bacterially expressed FOX-1. The Ponceau-stained blot is shown below for loading control. The position of the 45- kD FOX-1-specific band is indicated to the left. (B) fox-1::lacZ expression in adult XX and XO animals is confined to small subsets of cells in the head and the tail. The expression pattern in XXs is distinct from that in XOs.

Picture from Benard C et al. (2001) Development "The C. elegans maternal-effect gene clk-2 is essential for embryonic ...."

Northern and western analyses of clk-2 at all developmental stages (E, embryos; L1-L4, larval stages; A, young adults; glp-4(bn2ts) adults).

Picture from Hesselson D et al. (2006) J Cell Biol "Growth control by EGF repeats of the C. elegans Fibulin-1C isoform."

Figure 5. EGF2C and FIBC domains are redundant signals for localization to pharyngeal basal lamina. (left and middle) Heads of fbl-1(q771) L4 larvae; transgenes are noted in the figure. Arrowheads indicate pharyngeal basal lamina; anterior to the left. Arrows indicate fluorescence from the coinjection marker. Bars, 20 um. (left) 0.8-um confocal slices. (middle) Differential interference contrast image of the same animal. (A, F, L, O, and R) Constructs with either EGF2C or FIBC localize to pharyngeal basal lamina. (I) VENUS::FBL-1D not in pharyngeal basal lamina. (right) Anti-VENUS Western blots. Both full-length FBL-1 isoforms are predicted to migrate at 100 kD, but FBL-1D only accumulates an 50-kD degradation product. TG, extract from transgenic strain noted in the left column; WT, extract from wild-type animals not carrying transgene; VF, extract from animals carrying VENUS::FBL-1; Act, actin loading control. Arrows mark the predicted sizes of transgenic proteins. Arrowheads mark abundant degradation products.

Picture from Pispa J et al. (2008) BMC Dev Biol "C. elegans dss-1 is functionally conserved and required for oogenesis and larval ...."

dss-1 mRNA is expressed at different larval ages (L1-L4) and in adult worms (ya, young adult) as shown by RT-PCR. act-4, actin(M03F4.2).