Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Figure S1. Expression patterns of hlh-17::GFP and ptr-10::myrRFP reporter transgenes. (A-D) DIC (A,C) and fluorescence (B,D) images of an L1-stage animal expressing hlh-17::GFP. Expression in the head (A,B) and tail (C,D) is shown. Arrowheads in B indicate expression of GFP in some IL, OL and OLQ sheath and/or socket glia. (E,F) DIC (E) and fluorescence (F) images of an L3-stage animal expressing hlh-17::myrGFP. Arrowheads indicate the expression of myrGFP in motoneuron commissures. (G-L) Merged fluorescence and DIC images (G,I,K) and fluorescence images (H,J,L) of an adult animal expressing ptr-10::myrRFP. Expression in the head (G,H), vulva (I,J) and rectum (K,L) is shown. Arrowheads in H indicate the expression in sheath and socket glia of IL, OL (blue), and OLQ (yellow) sensilla, as well as in CEPsh glia (white).

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

(A) Merged DIC/fluorescence and (B) fluorescence image of wild-type (WT) adult C. elegans expressing dat-1::GFP in CEP neurons. White and yellow arrowheads indicate dorsal and ventral left dendrite tips, respectively. Scale bar: 5μm.

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Expression pattern of ptr-10::myrRFP reporter transgene. (G-L) Merged fluorescence and DIC images (G,I,K) and fluorescence images (H,J,L) of an adult animal expressing ptr-10::myrRFP. Expression in the head (G,H), vulva (I,J) and rectum (K,L) is shown. Arrowheads in H indicate the expression in sheath and socket glia of IL, OL (blue), and OLQ (yellow) sensilla, as well as in CEPsh glia (white).

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Figure 2. CEPsh glia are required for CEP neuron dendrite extension. (A) Merged DIC/fluorescence and (B) fluorescence image of wild-type (WT) adult C. elegans expressing dat-1::GFP in CEP neurons. White and yellow arrowheads indicate dorsal and ventral left dendrite tips, respectively. (C,D) Fluorescence images of at-1::GFP-expressing adult animals lacking either ventral (C) or dorsal (D) left CEPsh glia. (E,F) Fluorescence images of mls-2(ns156) (E) and vab-3(ns157) (F) adults, respectively, expressing dat-1::GFP. (G) Dendrite extension defects of strains of the indicated genotype. Scale bar: 5μm.

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

(A) The C. elegans head. In all figures, dorsal is up and anterior is left. Posterior regions of ventral CEPsh glia ensheath the ventral ganglion. (B,C) Fluorescence images of a wild-type adult expressing hlh-17::GFP in CEPsh glia (white arrowheads) and the T08G3.3::RFP ADF neuron reporter (yellow arrowheads). (B) Overlay. (C) ADF alone. White arrows, ADF axon. (F-N) Fluorescence (F,G) and merged DIC/fluorescence (H) images of wild-type adults expressing hlh-17::GFP and ptr-10::myrRFP. White and yellow arrowheads indicate dorsal and ventral CEPsh glia, respectively. Asterisks indicate non-CEPsh glia. Scale bars: 5 μm.

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Figure 1. mls-2 and vab-3 regulate the development of ventral and dorsal CEPsh glia, respectively. (A) The C. elegans head. In all figures, dorsal is up and anterior is left. Posterior regions of ventral CEPsh glia ensheath the ventral ganglion. (B,C) Fluorescence images of a wild-type adult expressing hlh-17::GFP in CEPsh glia (white arrowheads) and the T08G3.3::RFP ADF neuron reporter (yellow arrowheads). (B) Overlay. (C) ADF alone. White arrows, ADF axon. (D) Dendogram depicting similarity of HLH-17 to Olig and other human and C. elegans bHLH protein subfamilies. (E) C. elegans hlh-17 gene structure. Boxes, exons; shaped lines, introns; arrow, position of a previously predicted translation start site different from that described here (see Materials and methods); TTTTCAG, trans-splicing site; the bHLH domain is in blue. (F-N) Fluorescence (F,G,I,J,L,M) and merged DIC/fluorescence (H,K,N) images of wild-type (F-H), mls-2(ns156) (I-K) and vab-3(ns157) (L-N) adults expressing hlh-17::GFP and ptr-10::myrRFP. White and yellow arrowheads indicate dorsal and ventral CEPsh glia, respectively. Asterisks indicate non-CEPsh glia. Scale bars: 5 μm.

Picture from Yoshimura S et al. (2008) Development "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and ...."

Expression pattern of hlh-17::GFP reporter transgene. (A-D) DIC (A,C) and fluorescence (B,D) images of an L1-stage animal expressing hlh-17::GFP. Expression in the head (A,B) and tail (C,D) is shown. Arrowheads in B indicate expression of GFP in some IL, OL and OLQ sheath and/or socket glia. (E,F) DIC (E) and fluorescence (F) images of an L3-stage animal expressing hlh-17::myrGFP. Arrowheads indicate the expression of myrGFP in motoneuron commissures.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.