Picture from Sun H et al. (2023) MicroPubl Biol "Comparing engineered nuclear-localized reporter cassettes."

Figure 1. Comparing engineered nuclear-localized reporter cassettes:Fig 1. (A) Schematic of reporter cassette comparison. (B) Comparison of the different reporter cassettes for neuropeptide nlp-51. Analysis for nlp-51 GFP fluorescence in the RIP and AIM neurons is shown on the left, with representative images on the right. (C) Comparison of the different reporter cassettes for neuropeptide flp-20. Analysis for flp-20 GFP fluorescence in the LUA, PVR, PVC, and PLM neurons is shown on the left, with representative images on the right. For B and C, *P<0.05, **P<0.01, ***P<0.001 posthoc t-test with Tukey multiple comparison correction. The black bars on the bottom right corner of microscope images represent 10 µm. (D) Summary of T2A::3xNLS::GFP vs. SL2::GFP::H2B comparisons across 6 different neuropeptide genes. Expression is determined using the reporter alleles in combination with NeuroPAL (Yemini et al., 2021), and the expression pattern of the SL2::GFP::H2B cassette more closely matches scRNA-seq data (Taylor et al., 2021).