Figure 1 Effect of UNC-33L in dauer formation, lifespan, and locomotion of
unc-33 mutants:(A) Analysis of resistance to SDS in C. elegans dauers was measured as percent survival after treatment with 1% SDS. Plotted is the average of three replicas +/- the standard deviation, Statistical analysis was performed using one-way ANOVA and Tukey Post Hoc for pairwise comparisons. *** p-value &#
x2266;0.001.(B) Lifespan of C. elegans adult hermaphrodites was quantified during 22 days of adulthood. Plotted is the mean of a population of 60 animals obtained as three biological replicas of 20 nematodes per genotype. Data were analyzed using Oasis (Online Application for Survival Analysis) (Yang et al. 2011).(C) Thrashing rates in liquid media were quantified for nematodes 1, 4, 8, and 12 days of adulthood. The averages of thrashing rates are plotted +/- standard deviations. Statistical analyses were performed using two-way ANOVA. p-values &#
x2266;0.001 were obtained in comparisons among strains, among time, and a significant interaction effect (p-value &#
x2266;0.001) was detected between strain and time.