Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)).

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.

Picture from Dammermann A et al. (2008) J Cell Biol "SAS-4 is recruited to a dynamic structure in newly forming centrioles that is ...."

(A) High-resolution images of GFP:SAS-6 recruited to RFP-labeled sperm centrioles in embryos generated using the mating scheme in Fig. 1 A. Times (in seconds relative to cytokinesis onset) correspond to meiosis (-1,470 to -1,370 s), early (-1,180 to -1,156 s) and mid (-1,005 to -972 s) S phase, and late prophase (-281 to -267 s). (B) Normalized individual measurements of the integrated GFP:SAS-6 intensity coincident with sperm centriolar RFP signal.

Picture from Yang Y et al. (2007) Proc Natl Acad Sci U S A "Polycomb-like genes are necessary for specification of dopaminergic and ...."

Figure 2. Molecular structure of sor-3 and its expression pattern. (A) Cosmid C04E7 and the PCR fragment containing C04E7.2 can fully rescue the mutant defects in sor-3, whereas the neighboring cosmids F52D1, ZK1193, M02E1, and F38G1 cannot rescue the sor-3 mutant defects. The cDNA sequence of sor-3 was confirmed by sequencing RT-PCR products. Sixty-three base pairs in the predicted sor-3 gene are not present in our cDNAs. The length of the introns and exons is not to scale. (B) Protein sequence of SOR-3. sor-3(bp185) contains a glutamine to stop-codon mutation, deleting the C-terminal 62 aa. The MBT domain is marked in blue. (C) Alignment of the MBT domains found in SOR-3, mouse PcG protein Sfmbt, and fly PcG proteins Sex combs on midleg (SCM). The identical amino acids found in the SOR-3 MBT domain and at least in one other MBT domain are highlighted. (D and E) Expression of sor-3::gfp in developing embryos (D) and larvae (E). sor-3 is expressed in all cells examined. (F) SOR-3 is localized in both nucleus and cytoplasm but excluded from nucleolus.

Picture from Meng L et al. (2016) PLoS Genet "Regulation of Gap Junction Dynamics by UNC-44/ankyrin and UNC-33/CRMP through ...."

Fig 3. UNC-44 acts upstream of UNC-33. Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::unc-33(S)). (C) Overexpression of UNC-33(S) suppresses unc-44(lf) phenotypes. Each experiment was performed with N>200 animals at least three times. For transgenic animals the results shown here are generated from at least three independent lines. Data are shown as mean +- SD. Student's t-test, ** p<0.01. Scale bar: 10 um.

Picture from Ellefson ML et al. (2009) Mol Biol Cell "Kinesin-1 and cytoplasmic dynein act sequentially to move the meiotic spindle to ...."

Images of mCherry-histone (top row) and GFP: DHC-1 (bottom row) within a wild-type meiotic embryo are shown from a representative time-lapse sequence. The cortex has been highlighted in each image for clarity. 0 s is the start of rotation. GFP:DHC-1 was faintly localized across the meiotic spindle before spindle shortening (T = -120 s). 45-30 s before the start of spindle rotation, DHC-1:GFP began to accumulate on meiotic spindle poles and remained on meiotic spindle poles after spindle rotation (T = +60 s).

Picture from Madhu Bhoomi J et al. (2019) MicroPubl Biol

Figure 1. Effects of S. marcescens and S. epidermidis on egg laying and brood size in wild-type and dbl-1(nk3) populations. (A) Comparison of mean eggs laid between wild-type (WT) and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. The counts of number of eggs laid were reported only for plates with live animals. Error bars represent SEM. n = 7-10 surviving animals each. (B) Comparison of total brood size between wild-type and dbl-1(nk3) populations fed on E. coli OP50, S. marcescens, or S. epidermidis. Total brood sizes of animals that desiccated were censored. Error bars represent SEM. n = 7-10 surviving animals each. Within each genetic background, significant differences between the E. coli OP50-fed control and the pathogen-fed populations are marked with asterisks (*, p< 0.05, **, p< 0.001).

Picture from Motegi F et al. (2006) Dev Cell "Two phases of astral microtubule activity during cytokinesis in C. elegans ...."

Behaviors of plus ends of astral microtubules at the cell cortex. The green box in the schematic embryo indicates the region at the cortex that was magnified. Images of GFP-tubulin and GFP-EB1 were acquired with 0.5 s and 1 s exposure times, respectively. CR, contractile ring. Times (s) are with respect to the onset of posterior movement of the spindle. The scale bar is 5 um.

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

Figure 3. TAC-1 Is Dynamically Associated with the MTOC in C. elegans Embryos(A) Fixed wild-type, 1-celled embryos stained for MTs (left panels; [i], [iii], [v], [vii], and [ix]) and TAC-1 (right panels; [ii], [iv], [vi], [viii], and [x]). (i and ii) TAC-1 is enriched at the poles of the meiotic spindle. (iii and iv) TAC-1 is associated with the sperm centrosomes (arrows in [iv]). (v and vi) High TAC-1 accumulation at the centrosomes in metaphase. (vii and viii) Reduced TAC-1 accumulation in telophase; (ix and x) 2-cell stage. The scale bar represents 10 um.(B) Still images from a time-lapse movie of an embryo expressing GFP:TAC-1. The pictures show GFP:TAC-1 behavior from prophase in the 1-celled embryo (just after pronuclear-centrosome rotation) to the 2-cell stage. (0 s) prophase, (+85 s) metaphase, (+130 s) anaphase, (+140 s) telophase, (+700 s) early 2-celled embryo, (+805 s) interphase 2-celled embryo. The scale bar represents 10 um.(C) GFP:TAC-1 around the chromosomes on a metaphase plate of a multicellular embryo. The scale bar represents 5 um.