Picture from Yang L et al. (2005) Development "The roles of two C. elegans HOX co-factor orthologs in cell migration and vulva ...."

Figure 7. ceh-20 is expressed in nuclei of Q, P and V cells and their descendants. (A-C) Fluorescence micrographs of wild-type larvae carrying ceh-20::gfp. This plasmid rescued the cell migration defects (QL and QR descendants, BDU) and the multivulval defect of ceh-20(mu290) animals. Dorsal is upwards and anterior is towards the left. (A,B) At hatching, ceh-20 is expressed in the nuclei of ectodermal such as the Q cells, P cells, V cells and hyp7. The mesodermal precursor, M, also expresses ceh-20 robustly. (C) Six to eight hours after hatching, V cell daughters, hyp7 cells and P cells continue to express ceh-20. In this animal, the right side is shown. The descendants of QR have migrated anteriorly and are in the process of dividing (not visible by fluorescence). (D) In this L2 animal, P(1-11).p cells express ceh-20, although only P(3-8).p are shown. Ventral cord neurons between the Pn.p cells also express ceh-20::gfp.

Picture from Lopez ME et al. (2022) MicroPubl Biol

Figure 1 Effect of UNC-33L in dauer formation, lifespan, and locomotion of unc-33 mutants:(A) Analysis of resistance to SDS in C. elegans dauers was measured as percent survival after treatment with 1% SDS. Plotted is the average of three replicas +/- the standard deviation, Statistical analysis was performed using one-way ANOVA and Tukey Post Hoc for pairwise comparisons. *** p-value ≦0.001.(B) Lifespan of C. elegans adult hermaphrodites was quantified during 22 days of adulthood. Plotted is the mean of a population of 60 animals obtained as three biological replicas of 20 nematodes per genotype. Data were analyzed using Oasis (Online Application for Survival Analysis) (Yang et al. 2011).(C) Thrashing rates in liquid media were quantified for nematodes 1, 4, 8, and 12 days of adulthood. The averages of thrashing rates are plotted +/- standard deviations. Statistical analyses were performed using two-way ANOVA. p-values ≦0.001 were obtained in comparisons among strains, among time, and a significant interaction effect (p-value ≦0.001) was detected between strain and time.

Picture from Barriere A et al. (2012) PLoS Genet "Coevolution within and between regulatory loci can preserve promoter function ...."

Figure 1. The expression pattern of unc-47 is conserved despite divergent regulation. (A) Both C. elegans and C. briggsae promoters fused upstream of GFP in their endogenous trans-regulatory environments drive expression in all 26 GABAergic neurons (green). However, the C. briggsae promoter placed in the C. elegans trans environment additionally drives expression in SDQR and SDQL (blue). (B) Those cells were identified as SDQR/L based on their position and their characteristic projections. (C) For each combination of promoter and trans-regulatory environment, expression in SDQR and SDQL is presented. C. elegans is represented by straight lines, C. briggsae by wavy lines. Frequency of expression is represented by the width, and intensity of expression relative to D-type neurons by the height of black boxes. Number of individuals expressing and total number of individuals scored is indicated underneath. Measurements for independent strains are given in Figure S1. Differences in frequency of expression in SDQR are significant for all comparisons: C. elegans and C. briggsae promoters in C. elegans trans environment (p = 8.1610212), C. elegans and C. briggsae promoters in C. briggsae trans environment (p = 1.561028), C. elegans promoter in C. elegans and C. briggsae trans environments (p = 4.8610211), C. briggsae promoter in C. elegans and C. briggsae trans environments (p = 2.4610214). (D) Interpretation of the results presented in panel C. Both C. elegans and C. briggsae promoters in their endogenous trans environments drive low levels of expression in SDQR and SDQL, while disruption of the endogenous interactions either drives high levels of expression (C. briggsae promoter in C. elegans) or abolishes expression (C. elegans promoter in C. briggsae).

Picture from Beaster-Jones L et al. (2004) J Mol Biol "DNA binding and in vivo function of C.elegans PEB-1 require a conserved FLYWCH ...."

Figure 2. Cb-PEB-1 and Ce-PEB-1 are expressed in similar pattern. Fluorescence micrographs of 1.5-fold stage C. briggsae (A) and C. elegans embryos (B) stained with C. elegans PEB-1 antibodies (anterior is left). Staining is most abundant in pharyngeal nuclei in both C. briggsae and C. elegans (brackets). Fainter staining is visible in hypodermal cells and the hindgut in C. elegans and C. briggsae, although all staining was generally weaker in C. briggsae.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(C, C') Pgrl-2::GFP is expressed in the excretory duct and pore cells.

Picture from Barkoulas M et al. (2016) PLoS Genet "Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene ...."

(B-E) smFISH using a lin-3 probe in C. elegans N2 (data from [2]) (B), C. briggsae AF16 (C), C. angaria RGD1 (D) and O. tipulae CEW1 (E). Red arrow marks the position of the anchor cell.

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

(A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C.

Picture from Vazquez-Manrique RP et al. (2007) Genomics "The frataxin-encoding operon of Caenorhabditis elegans shows complex ...."

(a-c) Expression of F59G1.1::gfp in pharynx and gut (a), anal cells (b), and spermatheca (sph) (c).

Picture from Cui Y et al. (2009) J Biol Chem "Cadmium induces retinoic acid signaling by regulating retinoic acid metabolic ...."

bcmo-1::GFP expression in post-embryonic C. elegans. Images of untreated C. elegans (A, C, and E). L1 (A) and L4 (C) larvae are at 400x and 200x magnification, respectively. Adult C. elegans (E) is a composite of images acquired at 200x magnification. Arrowhead, indicates the location of a bcmo-1::GFP expressing embryo in a gravid adult nematode.

Picture from Loria PM et al. (2003) Curr Biol "Two neuronal, nuclear-localized RNA binding proteins involved in synaptic ...."

(B and C) Expression of promoter fusions in embryos at the (B) 300-cell stage and in (C) L1 larvae.