Picture from Xu L et al. (2001) Genetics "Caenorhabditis elegans MES-3 is a target of GLD-1 and functions epigenetically ...."

Figure 4. MES-3 localization in the germline. Gonad arms dissected from wild-type hermaphrodites were stained with affinity-purified rat anti-MES-3 and DAPI. The distal tip is indicated by an asterisk. Oocytes are in the bottom right corner. MES-3 is observed in the nuclei of the distal mitotic region, the proximal meiotic region, and oocytes. Its level is reduced to below detection in nuclei in early pachytene. Bar, 20 um.

Picture from Xu L et al. (2001) Genetics "Caenorhabditis elegans MES-3 is a target of GLD-1 and functions epigenetically ...."

Figure 3. Western blot and immunostaining of embryos by rat anti-MES-3 antibodies. (A) Affinity-purified rat anti- MES-3 antibody was used to probe wild-type (N2) or mes-3 (bn35) worm extract that had been size fractionated by SDS- PAGE and blotted to nitrocellulose membrane. One band of 90 kD was recognized by the anti-MES-3 antibody in the N2 lane but not in the bn35 lane. The same blot was probed with mouse anti-a-tubulin antibody as a loading control (bottom). (B) Wild-type or mes-3(bn35) embryos were stained with affinity-purified rat anti-MES-3 antibody and DAPI to visualize DNA. The stage of the embryo is indicated on the left. Four- cell embryo, staining is nuclear in all four cells; 30-50-cell embryo, MES-3 is present in all nuclei; Comma-stage embryo, faint staining is seen in all nuclei. The primordial germ cells Z2 and Z3 (one-third from right end of embryo) are more brightly stained. L1 larva, staining is detected primarily in the primordial germ cells Z2 and Z3 (right); bn35 4-cell embryo, nuclear staining is not detectable. Bar, 10 um. (C) Confocal images of wild-type embryos stained with rat anti-MES-3 and rabbit anti-histone to visualize chromosomes. Prometaphase, MES-3 is distributed in the nucleoplasm; metaphase and early anaphase, a portion of MES-3 is localized on chromosomes.

Picture from Xu L et al. (2001) Genetics "Depletion of a novel SET-domain protein enhances the sterility of mes-3 and mes-4 ...."

Figure 6. Immunolocalization of SET-2L in embryos and L1 larvae. Wild-type worms were stained with affinity-purified anti-SET-2L antibody and 4',6-diamidino-2-phenylindole (DAPI) to visualize DNA. During all stages of embryogenesis, SET-2L is localized in the nuclei of all cells. In L1 larvae, it appears to be present at higher levels in the primordial germ cells, Z2 and Z3 (arrow). (Bottom) SET-2L staining is not detectable in set-2l(RNAi) embryos. Bar, 10 um.

Picture from Xu L et al. (2001) Genetics "Depletion of a novel SET-domain protein enhances the sterility of mes-3 and mes-4 ...."

Figure 7. Immunolocalization of SET-2L in the germline. Extruded gonads of wild-type worms were stained with affinity-purified anti-SET-2L antibody and DAPI to visualize DNA. The distal region of the gonad, where germ nuclei divide mitotically and enter meiosis, is to the left, and oocytes are in the upper right. SET-2L is visible in the distal mitotic region of the gonad, undetectable in early pachytene nuclei, and present at high levels in mid- and late-pachytene nuclei. Its level decreases again in oocytes. Bar, 20 um.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(L) Pgrl-6::GFP is expressed in the PVT neuron.

Picture from Munro E et al. (2004) Dev Cell "Cortical flows powered by asymmetrical contraction transport PAR proteins to ...."

(J-L) Embryos fixed at metaphase and costained for NMY-2 and PAR-3.

Picture from Yin J et al. (2017) J Cell Biol "GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in ...."

(J-L") Confocal fluorescence images in wild type coexpressing GFP::GOP-1and markers of different organelles including Cherry::RAB-5 (J-J", early endosome), Cherry::RAB-7 (K-K", late endosome), or NUC-1::Cherry (L-L", lysosome). White arrowheads, vesicles with overlapping GFP and Cherry fluorescence; pink arrowheads, GFP- and Cherry-positive vesicles that are in closeproximity; yellow arrowheads, GFP-positive and Cherry-negative puncta. Quantification is shown at right (mean +- SD). Bars: (A-I') 10 um; (J-L") 5 um.

Picture from Tian Y et al. (2010) Cell "C. elegans screen identifies autophagy genes specific to multicellular ...."

(G-L) The epg-4 reporter is expressed ubiquitously in embryos (H) and widely in larvae (I). EPG-4::GFP colocalizes with the ER marker cherry::TRAM-1 (J-L). (G) Nomarski image of the embryo in H.

Picture from Pujol N et al. (2001) J Biol Chem "The Caenorhabditis elegans unc-32 gene encodes alternative forms of a vacuolar ...."

In situ hybridization images on embryonic, larval, and adult worms with vha-6 (I-L) are shown. vha-6 is expressed only in the intestine, from the embryonic coma stage (I) through larvae (J and K) and adult (L).

Picture from Mahoney TR et al. (2008) Proc Natl Acad Sci U S A "Intestinal signaling to GABAergic neurons regulates a rhythmic behavior in ...."

(G'-L') aex-2 is expressed in the GABAergic neurons AVL and DVB as well as in enteric muscles. (G and I) AEX-2::mCherry is detected in the nerve ring (NR, arrow), ciliary sensory processes (CA, open arrow), nerve cord (I), and head mesodermal cell (HMC, arrowhead). (H) AEX-2::mCherry is expressed in the intestinal muscle (IM, arrow) and anal depressor (AD, arrowhead). (J'-L') mCherry expressed under the aex-2 promoter is detected in AVL (J-L, arrow) and DVB (J''-L'', arrow) GABAergic neurons. AEX-2::mCherry signal does not significantly overlap with GABAergic GFP in the ventral nerve cord (J'-L'). (Scale bar, 20 um.) See SI Text for transgenes and clones.