Picture from Ogurusu T et al. (2015) Biochem Biophys Res Commun "The Caenorhabditis elegans R13A5.9 gene plays a role in synaptic vesicle ...."

Fig. 1. Expression of R13A5.9 in synaptic regions. (A-C) Co-localization of R13A5.9:GFP with synaptic vesicle marker SNB-1:DsRed-Monomer in the nerve ring (white arrow head), ventral nerve cord (white arrow), and tail neurons (black arrow). (A) GFP image. (B) DsRed-Monomer fluorescence image of the same field in (A). (C) merged image of (A) and (B). (D) Spinning disk confocal image of R13A5.9:GFP (green) expression superimposed on an image of SNB-1:DsRed-Monomer (red) expression; co-localization occurs in the dorsal nerve cord (white arrows). (E) Confocal images of a synapse in a dorsal nerve cord were collected at 0.2 um intervals in the z axis. Confocal images of R13A5.9:DsRed-Monomer (red) expression were superimposed on those of SNB-1:GFP (green) expression; co-localization was examined using an ImageJ plugin (Colocalization Analysis, Wright Cell Imaging Facility, Canada). From left to right, Pearson's correlation coefficients were as follows: 0.68, 0.74, 0.74, 0.68, and 0.52. Scale bars in (A-C) represent 10 um; scale bars in (D) and (E)represent 5 um and 1 um, respectively