Fig. 1. Expression of R13A5.9 in synaptic regions. (A-C) Co-localization of R13A5.9:GFP with synaptic vesicle marker SNB-1:DsRed-Monomer in the nerve ring (white arrow head), ventral nerve cord (white arrow), and tail neurons (black arrow). (A) GFP image. (B) DsRed-Monomer fluorescence image of the same field in (A). (C) merged image of (A) and (B). (D) Spinning disk confocal image of R13A5.9:GFP (green) expression superimposed on an image of SNB-1:DsRed-Monomer (red) expression; co-localization occurs in the dorsal nerve cord (white arrows). (E) Confocal images of a synapse in a dorsal nerve cord were collected at 0.2 um intervals in the z axis. Confocal images of R13A5.9:DsRed-Monomer (red) expression were superimposed on those of SNB-1:GFP (green) expression; co-localization was examined using an ImageJ plugin (Colocalization Analysis, Wright Cell Imaging Facility, Canada). From left to right, Pearson's correlation coefficients were as follows: 0.68, 0.74, 0.74, 0.68, and 0.52. Scale bars in (A-C) represent 10 um; scale bars in (D) and (E)represent 5 um and 1 um, respectively
Sub-cellular localization pattern for F42C5.9. F42C5.9 is present in the cell membrane, as well as filament-like structures and dense body-like structures. Bar 10 um.
(E) DIC image of the ALA and RID neurons (white arrows). As previously reported, ALA is posterior to RID [S2]. (F) Overlay of nlp-8 gfp reporter expression on DIC image. nlp-8 is expressed in ALA, as well as other neurons in the head [S3].