Picture from Wong, Amanda C et al. (2020) MicroPubl Biol

Figure 1: Signal intensity and sperm parameters in the UX993 strain. (A) Images and brightness of spermatid nuclei from UX993 and its parent strains. (B) Single perspective of a 3-D rendering generated from a z stack taken through the gonad. The brightfield image is overlaid with fluorescence. When imaged in vivo, UX993 sperm nuclei (red) are clearly visible, and the sperm populations in the oviduct, spermatheca, and uterus are easily distinguishable from one another. Female germline and oocyte nuclei are pseudocolored white. The cluster of sperm within the spermatheca is indicated by the white dotted line. (C) UX993 hermaphrodite fertility is comparable to that of wild-type hermaphrodites. Bar graphs show average progeny counts excluding those from the first 24 hours post-L4; error bars indicate 95% confidence intervals. ***, p<0.01; ns, not significant (Student's t test). (D) N2 and UX993 hermaphrodites have indistinguishable numbers of sperm. Bar graph shows average sperm counts in 24-hour post-L4 hermaphrodites; error bars indicate 95% confidence intervals. n=15-16. (E) UX993 hermaphrodite sperm have wild-type usage patterns: they are outcompeted by wild-type male sperm, and they outcompete competition-deficient comp-1 male sperm. Each dot represents one mating, and results for one representative repeat are shown. Gray lines indicate median values. **, p<0.001 (Kolmogorov-Smirnov test). Scale bars, 20 um.

Picture from Wong, Wan-Rong et al. (2021) MicroPubl Biol

Figure 1. The ALDH1A3(C174Y) variant and its C. elegans ortholog, alh-1(C172Y): (A) Domains and sequence conservation between human ALDH1A3 and its C. elegans ortholog, alh-1. The three domains in human ALDH1A3 are colored differently: the NAD binding domain is shown in blue, the catalytic domain in pink, and the oligomerization domain in yellow. Overall, the human ALDH1A3 is conserved with C. elegans ALH-1 at 77% of amino acid positions. The protein sequence alignment around the ALDH1A3 C174Y residue is highlighted in the bottom row. (B) In our C. elegans model, the alh-1 (C172Y) missense mutant displayed a significantly reduced fecundity. Approximately 20 animals were tested in each strain. Each dot represents one hermaphrodite. P< 0.0001 via non-parametric Mann-Whitney test.

Picture from Wong CH et al. (2023) MicroPubl Biol "Superoxide dismutase SOD-3 regulates redox homeostasis in the ...."

Figure 1. SOD-3 plays a key role in regulating oxidative stress in the intestine:(A) Intestine-specific rxRFP fluorescence intensity is elevated in the sod-3(tm776) background. Left: fluorescence intensity of intestine-specific rxRFP in sod-1(tm776), sod-5(tm1146), sod-2(ok1030), and sod-3(tm770). Right: representative fluorescence micrographs. Error bar represents s.e.m., **** represents p < 0.0001, and ns represents not significant, as determined by one-way ANOVA, followed by Tukey's multiple comparison analysis.(B) sod-2(ok1030) suppresses the sod-3(tm770)-dependent rxRFP fluorescence increase.(C) sod-1(tm776) suppresses the sod-3(tm770)-dependent rxRFP fluorescence increase.(D) The sod-1(tm776) and sod-5(tm1146) double mutant suppresses the sod-3(tm770)-dependent rxRFP fluorescence increase.(E) The sod-1(tm776), sod-5(tm1146), sod-2(ok1030), and sod-3(tm770) quadruple mutant elicits enhanced gut rxRFP fluorescence.(B-E) Error bar represents s.e.m., **** represents p < 0.0001, *** represents p < 0.001, and ns represents not significant as determined by one-way ANOVA, followed by Tukey's multiple comparison analysis.All worms were grown on E. coli OP50 at 20 °C prior to imaging.

Picture from Wong D et al. (2007) Genome Biol "Genome-wide investigation reveals pathogen-specific and shared signatures in ...."

Figure 4. Expression domains of common response genes and symptoms associated with infection. pF44A2.3::GFP expression in the (a) ventral nerve-cord, (b) hypodermis, (c-d) P12.pa pre-anal cells, (e-f) glial amphid socket cells, (g-h) excretory duct cell and (i) vulE or uv1 cells. Red fluorescence comes from the pcol-12::dsRED co-injection marker. In areas where both GFP and dsRED are expressed, yellow is observed. (j,k) Vacuoles (arrows) can be observed within intestinal cells of P. luminescens-infected adults (j), in which there is detectable expression of asp-4::GFP (k). Similar results were obtained with infected adults expressing asp-3::GFP. In contrast, no GFP expression or vacuolization was seen in the intestines of non-infected worms.

Picture from Wong YF et al. (2010) BMC Dev Biol "mab-31 and the TGF-beta pathway act in the ray lineage to pattern C. elegans male ...."

Figure 3 Expression pattern of mab-31. The gfp expression from mab-31::gfp transcriptional reporter is found in amphid sockets(arrows) (B) and phasmid sockets (arrowheads) (D) in a hermaphrodite. In the male tail, gfp signal is detected in structural cell processes of all sensory rays (F). The functional MAB-31::GFP protein is localized in multiple ray cell nuclei (H). Lateral view, left side upwards for A-D. Ventral view for E-H. Scale bar = 20 um.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.