Picture from Williams K et al. (2015) PLoS One "The tail domain is essential but the head domain dispensable for C. elegans ...."

Fig 4. Fluorescence micrographs of representative animals expressing rod-deleted IFA-2::GFP variants in wild-type genetic background. Scale bars are 25um. A) L4 stage with IFA-2H::GFP expressed in the epidermal ridges (r) and incorporation into FOs in the areas within the epidermis overlying muscle (m) is visible. Seam cells (s) do not express the tagged protein. B) Adult with IFA-2H::GFP expressed in the seam (s), expression in epidermal ridges (r) is not obvious. Uterine expression (u) includes both utse and uv cells. C) L4 stage with IFA-2T::GFP expressed at high levels in the epidermal ridges (r) but not seam cells (s). Incorporation into FOs in the areas of epidermis overlying muscle (m) is not detectable.

Picture from Williams K et al. (2015) PLoS One "The tail domain is essential but the head domain dispensable for C. elegans ...."

Fig 2. Fluorescence micrographs showing IFA-2::GFP variant localization in live adult transgene carrying lines. Scale bars for FO and ALM micrographs are 10um, for uterus micrographs the scale bars are 20 um. A) Full length IFA-2::GFP fusion protein localizes to epidermal FOs in rescued ifa-2::gfp; ifa-2(nc16) animals. The GFP-dependent fluorescence is detected in thin stripes oriented perpendicular to the long axis of the worm in regions of the epidermis adjacent to muscle. B) Full length IFA-2::GFP fusion protein localizes to uterine seam and to C) ALM in ifa-2::gfp; ifa-2(nc16). D, E, F) Localization of headless IFA-2H::GFP protein is indistinguishable from IFA-2::GFP. Genotype shown is ifa-2deltaH::gfp; ifa-2(nc16) G,H, I) Localization of IFA-2deltaT::GFP protein is indistinguishable from IFA-2::GFP. Genotype shown is ifa-2deltaT::gfp; ifa-2(+). J,K,L) Localization of IFA-2R::GFP protein is indistinguishable from IFA-2::GFP. Genotype shown is ifa-2R::gfp; ifa-2(+).

Picture from Williams K et al. (2015) PLoS One "The tail domain is essential but the head domain dispensable for C. elegans ...."

B) Same worm as in A, showing IFB-1::GFP expression. Note co-incidence of expression with IFA-2::GFP in fibrous organelles (fo), and uterine seam (arrow-head with black outline). Excretory canal shows IFB-1-expression (all-white arrowheads) that is not associated with IFA-2 co-expression. Punctuate autofluorescence of gut granules is also observable in this panel. Ectopic accumulations of IFB-1 in the epidermal ridges co-localize with accumulations of IFA-2::RFP at the same locations.

Picture from Williams K et al. (2015) PLoS One "The tail domain is essential but the head domain dispensable for C. elegans ...."

Fig 5. Fluorescence micrographs of adults co-expressing IFA-2::RFP and IFB-1::GFP. Scale bars are 50 um in panels A-C, 10 um in panels D-E. A)IFA-2::RFP is expressed in fibrous organelles (fo), uterine seam (arrow-head with black outline). Arrows indicate a region of brighter fluorescence that coincides with the edge of the muscle quadrant, the lower line of bright fluorescence also includes fluorescence associated with the touch neuron. B) Same worm as in A, showing IFB-1::GFP expression. Note co-incidence of expression with IFA-2::GFP in fibrous organelles (fo), and uterine seam (arrow-head with black outline). Excretory canal shows IFB-1-expression (all-white arrowheads) that is not associated with IFA-2 co-expression. Punctuate autofluorescence of gut granules is also observable in this panel. Ectopic accumulations of IFB-1 in the epidermal ridges co-localize with accumulations of IFA-2::RFP at the same locations. C) Enlargement and red/green overlay of a portion of anterior body showing co-ncidence of IFA-2::RFP and IFB-1::GFP expression. Arrows and arrowheads identify the same structures as seen in A and B. Both the co-localization of IFA-2 and IFB-1 in the touch-neuron (adjacent to lower arrow) and in ectopic accumulations is apparent. (The punctate pattern of the FOs is not in the plane of focus of this image.) D) Enlargement and red/green overlay of uterine seam region showing IFA-2::RFP and IFB-1::GFP expression. Co-localization of IFA-2 and IFB-1 in the uterine seam (arrow-heads with black outline), FOs (fo) and in ectopic accumulations is readily apparent. The IFB-1-only expressing excretory canal is indicated with the all-white arrowhead. E) 1,000x red/green overlay photomicrograph of FOs (fo) and adjacent touch neuron (arrowhead labeled tn) showing co-expressionof IFA-2::RFP and IFB-1::GFP expression. F) 1,000x red/green overlay photomicrograph of touch neuron (arrowhead labeled tn) showing co-expression of IFA-2::RFP and IFB-1::GFP, and excretory canal (arrowhead) that lacks IFA-2 expression.

Picture from Williams K et al. (2015) PLoS One "The tail domain is essential but the head domain dispensable for C. elegans ...."

A) Full length IFA-2::GFP fusion protein localizes to epidermal FOs in rescued ifa-2::gfp; ifa-2(nc16) animals. The GFP-dependent fluorescence is detected in thin stripes oriented perpendicular to the long axis of the worm in regions of the epidermis adjacent to muscle. B) Full length IFA-2::GFP fusion protein localizes to uterine seam and to C) ALM in ifa-2::gfp; ifa-2(nc16).

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(K) Pgrl-8::GFP is expressed in two OLL neurons.

Picture from Huang CY et al. (2012) PLoS One "C. elegans EIF-3.K promotes programmed cell death through CED-3 ...."

Figure 2. EIF-3.K protein expression. (A) Western blot analysis of EIF-3.K protein expression. Affinity-purified anti-EIF-3.K antibodies were used to probe a blot of embryonic extracts from wild-type and eif-3.K(gk126) worms (above). Equal loading of the two extracts was confirmed by anti-a tubulin antibodies (below). The sizes of molecular weight markers and the positions of EIF-3.K and a tubulin are indicated. (B-D) Images of an eif-3.K(gk126)mutant early embryo (B), a wild-type early embryo (C) and a wild-type newly hatched larva (D) that were co-stained with anti-EIF-3.K antibodies and DAPI. Merged images are also shown. Scale bar = 10 um.

Picture from Manil-Segalen M et al. (2014) Dev Cell "The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by ...."

(D-K) Electron micrographs of GFP::LGG-1 (D-H) or GFP::LGG-2 (J and K) embryos incubated with anti-LGG-1 (D and E) or anti-GFP (F-H, J, and K) antibodies revealing autophagosomes (D, F, and J), isolation membranes (E, G, and K) andoccasionally, the lumen of lysosomes (H). (D0-K0) Higher magnifications of the positive structures observed in (D-K). Scale bar represents 200 μm. (I) Quantification of the diameter of the autophagosomes and of the localization of gold beads are shown as mean ± SEM.

Picture from Li P et al. (2016) Neuron "Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite ...."

(J-K) GFP-tagged SER-1 is co-expressed with a cytosolic tdTomato in AVE in wild type (J), apb-3(ok429)(K) animals. Arrows point to the dendritic region of the AVE neuron. Scale bar is 10um.