Picture from Labrousse AM et al. (1998) Mol Biol Cell "Contribution of the GTPase domain to the subcellular localization of dynamin in ...."

Figure 1. Endogenous dynamin detected in C. elegans by immunofluorescence. A wild-type (N2) animal was stained with anti-dynamin antibodies. The immunofluorescence is concentrated in the nerve ring (nr) and along ventral (vc) and dorsal (dc) nerve cords. The punctate pattern in the pharynx corresponds to pharyngeal neurons (pn). Dynamin was also detected in sublateral neurons (sn). The inset shows that dynamin accumulates along the apical surface of intestinal cells (arrowhead). In this case, the worm was broken during the permeabilization procedure, which helped unmask the specific intestinal staining.

Picture from Humphrey JA et al. (2007) Curr Biol "A putative cation channel and its novel regulator: cross-species conservation ...."

For the nca-1 construct, the fluorescence pattern exhibited by a typical L3 hermaphrodite is shown. GFP expression is observed in a subset of neurons in the pharyngeal nervous (PN) system, in numerous unidentified nerve ring (NR) interneurons, and in a subset of motor neurons of the ventral nerve cord (VNC). Expression is also observed in a few cell bodies of the dorsorectal and lumbar ganglia (bracket). Scale bar represent 50 mm. We would like to thank A. Fire (Stanford University) for providing the plasmid pPD95.67 used to generate the nca-1 GFP reporter construct.

Picture from Zheng C et al. (2017) Mol Biol Cell "Distinct effects of tubulin isotype mutations on neurite growth in ...."

Figure S8. TBA-7 is expressed and acts cell-autonomously in the TRNs. (A) A schematicrepresentation of the tba-7::GFP reporter, containing a 1.2 kb promoter and the entire coding region.(B) The expression of tba-7::GFP in the TRNs, which are also labeled by mec-17p::RFP. Arrowspoint to the cell bodies of TRNs. The posterior intestine also showed strong GFP signal (the rightmostpanel). (C) The length of ALM-PN in tba-7(u1015 lf) mutants that carried a rescuing array thatexpressed wild-type tba-7 in the TRNs specifically.

Picture from Humphrey JA et al. (2007) Curr Biol "A putative cation channel and its novel regulator: cross-species conservation ...."

For the nca-2 construct (bottom), the fluorescence pattern exhibited by a typical L4 hermaphrodite is shown. Expression is observed in a few neurons of the pharyngeal nervous system (PN), in numerous unidentified NR interneurons, in most motor neurons of the VNC, and in neurons and muscle cells in the tail (arrow). Diffuse GFP expression is also observed in vulval muscles (VM) and diffusely in the intestine (INT). Scale bar represents 50 mm. We would like to thank A. Fire (Stanford University) for providing the plasmid pPD95.75 used to generate the nca-2 GFP reporter construct.

Picture from Humphrey JA et al. (2007) Curr Biol "A putative cation channel and its novel regulator: cross-species conservation ...."

Figure S3. Patterns of Expression of na/nca and unc-79. (A) Reporter constructs linking the promoters for each channel gene to green fluorescent protein (GFP). For the nca-1 construct (top), the fluorescence pattern exhibited by a typical L3 hermaphrodite is shown. GFP expression is observed in a subset of neurons in the pharyngeal nervous (PN) system, in numerous unidentified nerve ring (NR) interneurons, and in a subset of motor neurons of the ventral nerve cord (VNC). Expression is also observed in a few cell bodies of the dorsorectal and lumbar ganglia (bracket). For the nca-2 construct (bottom), the fluorescence pattern exhibited by a typical L4 hermaphrodite is shown. Expression is observed in a few neurons of the pharyngeal nervous system (PN), in numerous unidentified NR interneurons, in most motor neurons of the VNC, and in neurons and muscle cells in the tail (arrow). Diffuse GFP expression is also observed in vulval muscles (VM) and diffusely in the intestine (INT). Scale bars represent 50 mm. We would like to thank A. Fire (Stanford University) for providing the plasmids pPD95.67 and pPD95.75 used to generate the nca-1 and nca-2 GFP reporter constructs. (B) A reporter construct linking the unc-79 promoter to GFP shows multiple patterns of expression in C. elegans. In adults, the predominant pattern (top left) involves staining of one or two neurons in the head, with processes extending to the snout. Expression was rarely seen in an additional set of paired neurons in the tail (bottom left). In L2 and L3 animals (right), cells of the ventral nerve cord are seen exclusively.

Picture from Delattre M et al. (2006) Curr Biol "Sequential protein recruitment in C. elegans centriole formation."

One-cell-stage embryos during meiosis I, meiosis II, pronuclear (PN) formation (corresponding to early S phase), and mitosis, as indicated. In all figures, anterior is to the left, insets are approximately 6-fold magnified views of centrosomes, arrowheads indicate the focus of the panels where appropriate, and scale bars represent 10 mm; moreover, DNA is shown in blue. Arrow in Figures 1B points to the first polar body.(A-D) GFP-SPD-2 transgenic embryos stained with antibodies against SAS-4 (red and top insets) and GFP (green and bottom insets). Thirteen of 18 meiosis I embryos and 6 of 6 meiosis II embryos exhibited GFP-SPD-2 signal at centrioles; the signal was typically weaker in meiosis I (compare [A] and [B]). Note that GFP-SPD-2 is also present at the PCM during mitosis [5, 6].

Picture from Nonet ML et al. (1997) J Neurosci "Caenorhabditis elegans rab-3 mutant synapses exhibit impaired function and are ...."

Figure 2. RAB-3 expression in the C. elegans nervous system. Whole worms were fixed and stained with anti-RAB-3 and anti-synaptotagmin primary antibodies and visualized with FITC- or Cy3-conjugated antibodies. A, B, Lateral view of the head region of a wild-type adult hermaphrodite showing RAB-3 (A) and synaptotagmin (B) immunoreactivity in the nerve ring (NR), pharyngeal nervous system (PN), and SAB neuron axonal processes (arrows). C, D, Ventral view of the midsection of an adult hermaphrodite showing RAB-3 (C) and synaptotagmin (D) immunoreactivity in the ventral nerve cord (VC;arrow) and the ventral sublateral processes.E, F, Lateral view of the vulval region of an adult hermaphrodite illustrating the absence of RAB-3 (E) but the presence of synaptotagmin (F) immunoreactivity in the uv1 cells of the somatic gonad (uv1; arrow). The ventral cord is also visible (VC).

Picture from Winston WM et al. (2002) Science "Systemic RNAi in C. elegans requires the putative transmembrane protein ...."

Figure 4. SID-1::C-GFP reporter expression patterns. SID-1::C-GFP is expressed in (A) the procorpus (pc), metacorpus (mc), and terminal bulb (tb) of the pharynx; (C) intestine (i); (E) spermatheca (sp) and proximal somatic gonad (g); (G) sphincter muscles (sm), phasmid neurons (pn), and extreme posterior intestinal cells (ip), which is stronger compared to more anterior intestinal cells (i); and in (I) the excretory cell where it is localized throughout the tubular processes. Because the SID-1::C-GFP expression array is not integrated, mosaic expression patterns are observed. [In (C) a poorly expressing or not expressing intestinal cell is marked (i-).] (K) Cell periphery localization (arrow heads) of SID-1::C-GFP compared with (L) cytoplasmic GFP (myo-2::GFP) in deconvolved images representing 0.9 uM section of the pharynx. (B,D, F, H, and J) Accompanying DIC images. Scale bars, 10 uM.

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

Figure 7. unc-97 functions in muscle development. (A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C. (D-F): Loss-of-function phenotype of unc-97 as determined by RNAi. D and E show the progeny of animals subjected to unc-97 RNAi. Embryos either arrest and die at the twofold stage or die shortly after hatching (both categories were scored as arrested). Note that the pharynx develops relatively normally but that elongation of the rest of the animal is arrested. All these phenotypes are hallmarks of the pat phenotype (Williams and Waterston, 1994). Bottom, progeny of animals subject to RNAi with a control ds RNA derived from a new homeobox gene (Hobert, O., and G. Ruvkun, unpublished data), which displays no lethality.

Picture from Liu Q et al. (2010) J Cell Sci "Facioscapulohumeral muscular dystrophy region gene-1 (FRG-1) is an ...."

Figure 4. FRG-1 associates with body-wall muscle cell dense bodies, adjacent to PAT-3, and colocalizes with ALP-1. (A-F) Adult animals co-immunostained for FRG-1 (A,D, green) and PAT-3 (B,E, red) show FRG-1 present at all body-wall muscle dense bodies (C, arrowheads), whereas it is absent from M-lines (C, arrows). FRG-1 and PAT-3 show adjacent, partially overlapping signals at dense bodies when merged (C,C', arrowheads). (D-F) Confocal images of dense body immunostaining. (G-I) Double transgenic animals for Pmyo-3::frg-1::yfp and Pmyo-3::alp-1alpha::cfp show that expression patterns for FRG-1 (G) and ALP-1 (H) are precisely colocalized in the image merge (I and I'). (J-L) Mutant C. elegans strain containing a homozygous partial deletion for atn-1 was co-immunostained for FRG-1 (J, green) and PAT-3 (K, red), showing disrupted FRG-1 localization (L). (M) Model depicting FRG-1 localization in dense bodies with the analogous vertebrate components (based on Moerman and Williams, 2006; Fluck et al., 2002). Scale bars: 20 μm. DGC, dystrophin-glycoprotein complex; IRC, integrin receptor complex.