Picture from Chao LF et al. (2017) PLoS Genet "An SMC-like protein binds and regulates Caenorhabditis elegans ...."

Fig 3. SMCL-1 expression and protein features. (A) Adult hermaphrodites from wild-type (WT) and a straincarrying the map::smcl-1 transgene driven by endogenous smcl-1 5' and 3' elements. A section of thegermline is shown, imaged by DIC to show structures and fluorescent microscopy to detect mVenusexpression from the MAP tag. Arrowheads denote the first four oocytes. (B) A typical SMC protein folds backon itself at a hinge domain, bringing coil regions together and creating a "head domain" (yellow) from ATPasedomains in the N- and C-termini. SMCL-1 lacks predicted coil and hinge domains, but has N- and C-terminalATPase domains that may be capable of forming a head domain (purple). (C) SMC head domain and theATPase cycle, showing binding of ATP (red circle), ATP-dependent engagement of heads from two SMCproteins, and disengagement upon ATP hydrolysis. (D) SMCL-1 amino acid sequence aligned to C. eleganscondensin SMC proteins. Shown are regions surrounding three conserved motifs found in SMCs and relatedATPases: the Walker A motif, ABC transporter signature motif, and Walker B motifs, and their consensussequences. SMCL-1 shares a conserved Walker A motif, but differs from consensus signature motif andWalker B motif at residues shown in red. Asterisk denotes catalytic amino acid required for ATP hydrolysis.x = any amino acid and h = hydrophobic amino acid.

Picture from Kelley CA et al. (2018) Mol Biol Cell "The myosin light-chain kinase MLCK-1 relocalizes during Caenorhabditis ...."

(A) Confocal maximum intensity projections ofan animal expressing GFP under the mlck-1 promoter. Strong GFP expression isconsistently seen in the spermatheca (ii) and pharynx (iv). Expression is alsoseen in the anal sphincter (i) and vulval cells (iii) however the expression patternin these tissues is more variable. Note images in (i and iii) are not from the sameanimal shown in (A).

Picture from McLachlan IG et al. (2018) PLoS Genet "A neuronal MAP kinase constrains growth of a C. elegans sensory dendrite ...."

(C) Schematic showingquantification of superfolderGFP-MAPK-15 enrichment at dendrite ending. Wild-typeanimals expressing flp-8pro:mCherry (URX) and flp-8pro:superfolderGFP-MAPK-15(URX) were imaged. Fluorescence intensity of a 2-um diameter circle (yellow) in asingle optical plane at the dendrite ending (i, ii) or middle (iii, iv) was calculated andbackground fluorescence was subtracted to yield a corrected intensity (yellow numbers).Tip enrichment was calculated as the ratio of corrected intensities at the tip vs middle (i iii, ii iv). Relative enrichment of the GFP signal was calculated by first normalizing tomCherry (i ii, iii iv) to correct for local differences in cell volume, and thencalculating the ratio of these normalized values ((i ii) (iii iv)). (D) Tip enrichmentof superfolderGFP-MAPK-15 and mCherry. Mean +- SD: GFP, 6.3 +- 3.9, mCherry, 1.7 +-0.7. n = 20. Colored bars, individual dendrites.

Picture from Anderson C et al. (2012) PLoS Genet "SLI-1 Cbl inhibits the engulfment of apoptotic cells in C. elegans through a ...."

Figure S1 Expression pattern of Psli-1::gfp. gfp was expressed under control of the sli-1 promoter. i, iii, v, vii and ix show fluorescence images and ii, iv, vi, viii and x show accompanying DIC images. i and ii, embryo at gastrulation; iii and iv, embryo at 1K-fold stage; v and vi, L1 head; vii and viii, L1 body; ix and x, L4 gonad with arrowheads showing DTC. Bar= 5 microns.

Picture from Anderson C et al. (2012) PLoS Genet "SLI-1 Cbl inhibits the engulfment of apoptotic cells in C. elegans through a ...."

Figure 3. Expression pattern of SLI-1::GFP under control of the sli-1 promoter. SLI-1::GFP was expressed under the control of its own promoter in an extrachromosomal array. (A) Images of morphologically normal animals expressing the SLI-1::GFP transgene. i, iii and v show fluorescence images and ii, iv and vi show accompanying DIC images (i-iv, embryos; v-vi, L4 larvae). Arrowheads in i-iv indicate positions of unengulfed cell corpses. In image iii corpses are not visible. Arrowheads in v and vi indicate the DTC. (B) Images of morphologically abnormal embryos expressing the SLI-1::GFP transgene. i, iii and v show fluorescence images and ii, iv and vi show accompanying DIC images. Arrowheads indicate positions of unengulfed cell corpses. Bar = 5 microns.

Picture from Anderson C et al. (2012) PLoS Genet "SLI-1 Cbl inhibits the engulfment of apoptotic cells in C. elegans through a ...."

Expression pattern of SLI-1::GFP under control of the sli-1 promoter. SLI-1::GFP was expressed under the control of its own promoter in an extrachromosomal array. (A) Images of morphologically normal animals expressing the SLI-1::GFP transgene. i, iii and v show fluorescence images and ii, iv and vi show accompanying DIC images (i-iv, embryos; v-vi, L4 larvae). Arrowheads in i-iv indicate positions of unengulfed cell corpses. In image iii corpses are not visible. Arrowheads in v and vi indicate the DTC. Bar = 5 microns.

Picture from Soukas AA et al. (2009) Genes Dev "Rictor/TORC2 regulates fat metabolism, feeding, growth, and life span in ...."

Tissues expressing rict-1 were determined by injecting wild-type animals with a transgene expressing RFP under the rict-1 promoter. I, II, IV, head neurons; III, ventral cord neurons; V, pharynx; VI, VII, body wall muscle; VIII spermatheca; and IX, intestine.

Picture from Le Bot N et al. (2003) Curr Biol "TAC-1, a regulator of microtubule length in the C. elegans embryo."

TAC-1 Is Dynamically Associated with the MTOC in C. elegans Embryos(A) Fixed wild-type, 1-celled embryos stained for MTs (left panels; [i], [iii], [v], [vii], and [ix]) and TAC-1 (right panels; [ii], [iv], [vi], [viii], and [x]). (i and ii) TAC-1 is enriched at the poles of the meiotic spindle. (iii and iv) TAC-1 is associated with the sperm centrosomes (arrows in [iv]). (v and vi) High TAC-1 accumulation at the centrosomes in metaphase. (vii and viii) Reduced TAC-1 accumulation in telophase; (ix and x) 2-cell stage. The scale bar represents 10 um.

Picture from McLachlan IG et al. (2018) PLoS Genet "A neuronal MAP kinase constrains growth of a C. elegans sensory dendrite ...."

S3 Fig. A functional GFP-MAPK-15 is enriched at the dendrite ending.(A) Wild-type or mapk-15 animals with or without flp-8pro:superfolderGFP-MAPK-15were synchronized as two-day adults and dendrite and nose lengths were measured.Colored bars, individual animals; black bars, population averages. n = 50 in all cases. (B)mapk-15 mutant animal expressing flp-8pro:mCherry (URX) and flp8pro:superfolderGFP-MAPK-15 (URX). Arrow, dendrite ending. (C) Schematic showingquantification of superfolderGFP-MAPK-15 enrichment at dendrite ending. Wild-typeanimals expressing flp-8pro:mCherry (URX) and flp-8pro:superfolderGFP-MAPK-15(URX) were imaged. Fluorescence intensity of a 2-um diameter circle (yellow) in asingle optical plane at the dendrite ending (i, ii) or middle (iii, iv) was calculated andbackground fluorescence was subtracted to yield a corrected intensity (yellow numbers).Tip enrichment was calculated as the ratio of corrected intensities at the tip vs middle (i iii, ii iv). Relative enrichment of the GFP signal was calculated by first normalizing tomCherry (i ii, iii iv) to correct for local differences in cell volume, and thencalculating the ratio of these normalized values ((i ii) (iii iv)). (D) Tip enrichmentof superfolderGFP-MAPK-15 and mCherry. Mean +- SD: GFP, 6.3 +- 3.9, mCherry, 1.7 +-0.7. n = 20. Colored bars, individual dendrites.

Picture from Edgley, Mark L. et al. (2021) MicroPubl Biol

Figure 1. Chromosome III in the qC1 balancer: (A) Breakpoint connections deduced by aligning split reads at the breakpoints. Identical strands indicates that the sequence on the left is connected directly to the sequence on the right, while different strands denotes a change of strand (inversion). Genes affected by the breakpoints are listed in the third column. (B) A schematic representation of chromosome III deduced for qC1 is shown. Each arrow represents the chromosomal segment of the reference sequence with the corresponding coordinates listed. The direction and color of each arrow represent the strand/direction of the sequence within the original reference chromosome. For simplicity of visualization, the complex rearrangement around 6.6Mb is shown separately. (C) A further simplified schematic representation of the chromosome rearrangements in qC1. Only the genomic segment represented in red is inverted relative to the wild-type chromosome III. Genes at breakpoints are indicated and the asterisks linked with the black wavy arrow represent the complex structural variation near 6.6Mb.