Picture from White, Rebekah J et al. MicroPubl Biol

(a) Location of the study sites around Iracambi, Minas Gerais State, in the southeast of Brazil (modified from Google Earth). Right: photograph of characteristic vegetation of each study site (RF, EU and GR); (b) Microfauna minitrap setup using 15 ml Falcon tube and stockings; (c) Microfauna isolated from three subsystems. Left and middle: GR, image taken using Leica MC170 HD. Right: Images taken using Swift Instruments International Stereo M23 and smartphone. Right top: RF, likely plant parasitic Meloidogyne sp. third-stage juvenile. Right middle: RF, likely predatory Tripyla sp. Right bottom: GR, likely free-living Alaimidae family.

Picture from White OR et al. (2024) MicroPubl Biol "Comparison of avoidance assay techniques to determine the response to 1-octanol ...."

Figure 1. The extent of avoidance of and adaptation to 1-octanol depends on the method of exposure to the odorant: (A) Time to response to an eyelash hair dipped in 10% 1-octanol for wild type (WT), kqt-2 knockout, and unc-25 Amsh glia knock down. N = 60 each. (B) Same as in A but the nematodes were exposed to 10 μl of 10% 1-octanol contained in a p10 pipette tip. N = 45 each. (C) The time to response to 10% 1-octanol on an eyelash hair was plotted against the order in which each nematode was tested within the same plate for WT, kqt-2 knockout, and unc-25 Amsh glia knock down. Each point represents an average of 4 animals. (D) Same as in C, but the nematodes were exposed to 10 μl of 10% 1-octanol contained in a p10 pipette tip. Averages are from 4 animals each. (E and F) Same as in C and D, respectively, but using 30% 1-octanol. Each point represents the average of 4 animals. (G) Adaptation to repetitive exposures to 10% 1-octanol on an eyelash hair for WT, kqt-2 knockout, and unc-25 Amsh glia knock down. The time to response was determined following exposure of the same nematode to 1-octanol 5 consecutive times (# trials) with at least 30 seconds interval in between exposures. (H) The comparison of the time of response to 1-octanol in the first versus the fifth trials (data from G) are shown here for the same strains. N = 15, 14, and 15 for WT, kqt-2 knockout, and unc-25 Amsh glia knock down, respectively. Data are shown as individual data points and mean +/- SE in panels A, B, and H, and as mean +/- SE in panels C-G. Statistics were by ANOVA with Tukey correction, **** denotes p < 0.0001, ns stands for non-statistically significant.

Picture from Kwan CS et al. (2008) BMC Physiol "TRPM channels are required for rhythmicity in the ultradian defecation rhythm of ...."

A-D: gtl-1 is expressed in the intestine. The intestine is marked by white arrows in panels A-D and a black arrow in panel E. Panels A-D show transgenic animals carrying GFP fused to part of the gtl-1 gene created as below. A, expression in the intestine (white arrows) of an adult. B, a closer view of the intestine (white arrow) of a young adult. C an oblique section in which GFP expression in the lining of the intestine (white arrow) is clear. D, expression in the intestines (white arrows) of two three-fold embryos.

Picture from Chiang JT et al. (2006) J Exp Biol "Evolution of pharyngeal behaviors and neuronal functions in free-living soil ...."

(I) Perinuclear expression of Pslo-1::SLO-1::GFP in the M4 neuron. The white arrows point to the M4 nucleus. The white arrowhead points to GFP signal surrounding the M4 nucleus.

Picture from Lamont LB et al. (2004) Dev Cell "FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans ...."

(A) FBF-1 staining in wild-type germline. White arrowheads highlight the distal and proximal boundaries of staining.

Picture from Yau DM et al. (2003) Proc Natl Acad Sci U S A "Identification and molecular characterization of the G alpha12-Rho guanine ...."

Figure 3. Expression of CeRhoGEF or Gpa-12 in C. elegans. (A) Expression of CeRhoGEFp::EGFP in the neurons in the head (white arrows). (B) Expression of CeRhoGEFp::EGFP in lumbar ganglia in the tail (white arrows). (C and D) DiI staining (shown in red) of amphid neurons in the head (C) or phasmid neurons in the tail (D). (E and F) GFP and DiI merged images show colocalization of CeRhoGEFp::EGFP at the amphid neurons in the head (E) or the phasmid neurons in the tail (F). (G and H) Diagram of CeRhoGEFp::EGFP expression in the head region (G) or tail region (H). (I) CeRhoGEFp::EGFP expression in the PLML and PLMR touch neurons (white arrows). (J) CeRhoGEFp::NLS::GFP expression in the ventral cord motor neurons (white arrows). (K) Possible expression in a cluster of neurons in the head (white arrow). (L) Gpa-12p::NLS::GFP expression in the ventral cord motor neurons (white arrows). (Scale bar: 100 um.)

Picture from Karakuzu O et al. (2009) Development "MIG-32 and SPAT-3A are PRC1 homologs that control neuronal migration in ...."

(A) An otherwise wild-type animal expressing Pnlp-1gfp in the HSN neurons. HSN cell bodies are indicated with a large white arrowhead. The position of the vulva is indicated. Small white arrowheads highlight the axons of the two HSN neurons, which proceed along the ventral body wall to the head.

Picture from Lamont LB et al. (2004) Dev Cell "FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans ...."

(E) FBF-2 staining in wild-type germline. White arrowheads highlight the distal and proximal boundaries of staining.

Picture from Yau DM et al. (2003) Proc Natl Acad Sci U S A "Identification and molecular characterization of the G alpha12-Rho guanine ...."

Expression of CeRhoGEF in C. elegans. (A) Expression of CeRhoGEFp::EGFP in the neurons in the head (white arrows). (B) Expression of CeRhoGEFp::EGFP in lumbar ganglia in the tail (white arrows). (C and D) DiI staining (shown in red) of amphid neurons in the head (C) or phasmid neurons in the tail (D). (E and F) GFP and DiI merged images show colocalization of CeRhoGEFp::EGFP at the amphid neurons in the head (E) or the phasmid neurons in the tail (F). (G and H) Diagram of CeRhoGEFp::EGFP expression in the head region (G) or tail region (H). (I) CeRhoGEFp::EGFP expression in the PLML and PLMR touch neurons (white arrows). (J) CeRhoGEFp::NLS::GFP expression in the ventral cord motor neurons (white arrows). (K) Possible expression in a cluster of neurons in the head (white arrow). (Scale bar: 100 um.)

Picture from Au C et al. (2009) PLoS One "SMF-1, SMF-2 and SMF-3 DMT1 orthologues regulate and are regulated ...."

A, SMF-1::GFP strongly localizes to the anterior and posterior intestine (solid white arrowheads), to the anchor cell (hollow arrowhead) and to head neurons (white arrows). smf-1::GFP and SMF-1::GFP reveal expression of smf-1 gene in rectal gland cells (B,C, black asterisks), in the uterus (uv1, uv2, utse syncytium, D,E, solid white asterisks) as well as in the adult spermatheca (F) and the L1 hyp7 epidermis (G, white arrowheads). Dotted lines outline the cuticle of the worm. Hollow asterisks indicate position of fertilized embryos. Hollow arrowheads indicate position of the vulva. Scale bars are 5 um.