Figure 3. Expression of
osm-7::GFP in the hypodermis. Nomarski (A and C) and fluorescence (B and D) images of two adult animals are shown. (A and B) The head region of one animal, with the anterior and posterior bulbs of the pharynx marked by closed arrows. Hypodermal cells expressing GFP are visible along the edges of the animal, surrounding the pharynx (B). (C and D) The midsection of another animal, with GFP-expressing hypodermal cells. Both images are at the top focal plane of the animal, so that the hypodermis is in focus but the intestine and other internal structures are not visible. The seam cells, which are not readily apparent in C, become visible as non-GFP cells along the center of the animal (open arrows) in D.