Figure 1. Phenotypic characterization of
as46 and its causative mutation: A. Hermaphrodite self-fertility is significantly reduced compared to wild-type N2 hermaphrodites (p<0.0001).B.
catp-4(
as46);
him-5 male fertility is significantly reduced compared to wild-type N2 males when mated to
fog-2(
oz40) hermaphrodites (p<0.0001).Each dot on the graph represents the total number of progeny produced by one individual hermaphrodite (n≥10). Error bars represent standard error (SEM).C. DAPI staining of
catp-4(
as46) and wild-type N2 hermaphrodites at L4+6 hrs L4+24 hrs. The spermathecae are marked by circles. Presence of sperm in the uterus (indicated by an arrow) is only observed in
catp-4(
as46) hermaphrodites. Scale bar = 20µmD. Morphology of spermatids and activated spermatozoa from
him-5(
e1490) control males and
catp-4(
as46);
him-5(
e1490) males. Scale bar = 10µmE. Aspect ratio comparison of
him-5(
e1490) sperm and
catp-4(
as46);
him-5(
e1490) sperm (n=140). Error bars represent standard error (SEM).F. Schematic of the
catp-4 genomic locus indicating the location of the
as46 mutation in the fourth exon. Below is an alignment of C. elegans CATP-4 with Na+/K+-ATPases from other organisms. The conserved glycine residue that is changed from glycine (G) to arginine (R) in the
catp-4(
as46) mutant is marked with a black rectangle.