Figure 3. Expression of SPR-2::GFP. All L3 stage. (A) Nomarski photomicrograph. The pi cells (23) are indicated with arrowheads. (B) GFP fluorescence in the nuclei of pi cells of the same hermaphrodite shown in A. (C) GFP fluorescence in the nucleus of the anchor cell (marked with arrowhead) and other gonadal cells. (D) GFP fluorescence in intestinal nuclei.
(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.
Expression pattern of T22A3.3 (H) as determined by promoter-GFP reporter constructs. (H) Expression of T22A3.3 is seen in a set of neurons in the head, including AWB.
(F-H) DAF-16 becomes more cytoplasmic with age, and nuclear localization of PQM-1::GFP decreases significantly with age. Data are represented as mean +/- SEM (G and H).
(A) and (C): DIC images of the embryos shown in (B) and (D). E-H. At postembryonic stages, epg-11::gfp is expressed in pharyngeal cells (E,F, arrows) and vulval cells (G,H, arrow). (E) and (G): DIC images of the animals shown in (F) and (H).