Picture from Zhao Z et al. (2004) J Mol Biol "Expression analysis of ABC transporters reveals differential functions of ...."

Pgp-14 (F22E10.3) gave expression in the anterior pharynx until half way along the first bulb.

Picture from Weng JW et al. (2024) MicroPubl Biol "Adult-specific collagen COL-19 is dispensable for contact-mediated mate ...."

Figure 1. Characterization the role of col-19 in contact-mediated mate recognition:(A) Quantification of COL-19::GFP (kaIs12) fluorescent signal with animals at L4 stages and adults. One-way ANOVA with Bonferroni correction and p values indicated. (B) Images of COL-19::GFP hermaphrodites treated by RNAi. Scale bar = 100 μm. (C) Quantification of the fluorescence intensity of COL-19::GFP hermaphrodites treated by RNAi. t test with p values indicated. (D) Genomic sequences of col-19 putative null mutants generated by CRISPR/Cas9 STOP-IN method. (E) Percentage of male leavers in the male retention assay. The dots represent 15-20 males in one replica. Error bar = S.E.M. One-way ANOVA with Bonferroni correction for statistical comparison and p values indicated.

Picture from Li Y et al. (2016) J Cell Biol "The lysosomal membrane protein SCAV-3 maintains lysosome integrity and adult ...."

(K-S) Confocal fluorescent images of wild type expressing LMP-1::sfGFP (K-R) orLMP-2::sfGFP (S). (T) The mean total fluorescence intensity of LMP-1::sfGFP in different cell types was measured, and the normalized florescence intensityis shown. At least 15 animals were scored in each cell type. Data are shown as mean +- SD. One-way ANOVA with Tukey's posttest was performed tocompare all the other datasets with hypodermis. *, P < 0.05; **, P < 0.0001. Bars, 10 um.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Symmetric expression of LET-19 in the T-cell division. Expression of let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.

Picture from Yoda A et al. (2005) Development "Components of the transcriptional Mediator complex are required for asymmetric ...."

Figure 5. Symmetric expression of LET-19 and DPY-22 in the T-cell division. Expression of dpy-22::GFP (A,B) and let-19::GFP (C,D) in the T cell. The T cell is in telophase in B and D. Anterior is towards the left, ventral towards the bottom.

Picture from Rennich BJ et al. (2023) MicroPubl Biol "Low-Density Lipoprotein Receptor LRP-2 regulates GLR-1 glutamate receptors ...."

Figure 1. LPR-2 regulates GLR-1 and glutamatergic behavior:(A) Response to nose-touch in WT, glr-1(n2461), lrp-2(gk556942) (6x backcrossed) and lrp-2(lst464) (2x backcrossed) (n=16-37 per genotype). Percent Responding (Mean +/- SEM) is shown. One-way ANOVA with Sidak's Multiple Comparison Test: **p<0.0001 vs WT. (B) Spontaneous locomotion reversal frequencies are shown for animals expressing constitutively-active GLR-1, GLR-1(A/T) (nuIs80), in the absence or presence of lrp-2(gk556942). Average Reversals/min +/-SEM are shown (n=36). Student's t test: **p<0.0001. (C-D) Representative images of SEP::mCherry::GLR-1 (akIs201) in the anterior (C) and posterior (D) VNC of AVA in WT and lrp-2(gk556942) mutants. Quantification of mCherry (total GLR-1) and SEP (surface GLR-1) fluorescence in the indicated genotypes are shown below the images. (n=37-57). Average fluorescence (Norm.) +/- SEM are graphed. Student's t test: **p<0.0001.

Picture from Borbolis F et al. (2024) MicroPubl Biol "MEC-12/alpha tubulin regulates mitochondrial distribution and mitophagy ...."

Figure 1. MEC-12/alpha tubulin regulates mitochondrial morphology and mitophagy under basal conditions and oxidative stress: (A) Single-cell RNA sequencing data confirming the pan-neuronal expression pattern of the mec-12 gene (data retrieved from https://www.cengen.org). (B) The amino acid sequence of MEC-12 with indicated mutations: acetylated lysine residue (red), D69N (u76), G144S (e1607), H192Y (e1605), G354E (u241), E425K (u63).(C) Transgenic animals expressing the fluorescent protein GFP fused with MEC-12 under the endogenous mec-12 promoter. The GFP::MEC-12 protein displays neuronal localization. Scale bar, 100μm. (D) Quantitative analysis of GFP::MEC-12 accumulation in neurons with age (ns P>0.05; ** P<0.001; **** P<0.0001; One-way ANOVA). (E) Representative confocal microscopy images of wild type (wt) and mec-12(e1605) mutants co-expressing mitochondria-targeted GFP and cytosolic mCherry in GABAergic motor neurons under control conditions and after exposure to 2mM paraquat. Scale bars, 20μm (F) Measurement of mitochondrial size in wt and mec-12(e1605) mutants, under control and paraquat-induced oxidative stress conditions (ns P>0.05; * P<0.01; **** P<0.0001; One-way ANOVA). (G) Effects of paraquat treatment on axonal mitochondrial number in wt and mec-12(e1605) mutants (** P<0.001; **** P<0.0001; One-way ANOVA). (H) Increase in GFP::MEC-12 levels upon paraquat treatment, indicating stress-induced up-regulation (head region; Scale bars, 100μm). (I) Quantitative analysis of GFP::MEC-12 protein levels in response to paraquat treatment (* P<0.01; unpaired t-test). (J) Representative fluorescent images of wt and mec-12(e1605) transgenic nematodes expressing mtRosella under the pan-neuronal promoter unc-119 treated with paraquat. Scale bars, 100μm. (K) MEC-12 is required for mitophagy induction (reduced GFP/DsRed ratio) upon paraquat treatment (ns P>0.05; **** P<0.0001; One-way ANOVA). (L) Representative fluorescent images of axonal mtRosella signals were obtained using wt and mec-12(e1605) transgenic nematodes, which express mtRosella pan-neuronally. Scale bars, 20μm. (M) The increased mtRosella ratio of mec-12(e1605) mutants suggests diminished basal mitophagy in axons (**** P<0.0001; unpaired t-test). (N) Mitophagic flux is reduced in the axons of mec-12(e1605) mutant nematodes (** P<0.001; unpaired t-test).

Picture from Rutkowski R et al. (2011) PLoS Genet "Regulation of Caenorhabditis elegans p53/CEP-1-dependent germ cell apoptosis ...."

(A) Germlines from young adult hermaphrodites were dissected and processed for immunofluorescence using an anti-CEP-1 antibody (green). DNA was visualised by DAPI staining (blue). All germlines are orientated the same way with the distal end to the left and the proximal end to the right.