Picture from Warnhoff K et al. (2017) PLoS Biol "The Nuclear Receptor HIZR-1 Uses Zinc as a Ligand to Mediate Homeostasis in ...."

Fig 4. Zinc promoted nuclear accumulation of HIZR-1 in animals. hizr-1(am286lf) animals expressing full-length HIZR-1(1-412 WT)::GFP or HIZR-1(1-412 D270N GF)::GFP were cultured with no supplemental metals or supplemental zinc or copper. (A-C) Representative images show midbody region; the intestines are outlined, arrowheads indicate representative GFP-positive nuclei, and scale bars are approximately 10 um. The correspondence of GFP fluorescence and intestinal cell nuclei was established by alternating between using differential interference contrast microscopy to visualize and identify nuclei based on morphology andlocation and fluorescence microscopy to visualize GFP. The punctate fluorescence in panel A appears to be autofluorescence derived from the gut granules, lysosome-related organelles that accumulate fluorescent material, and is not likely to result from HIZR-1(1-412 WT)::GFP expression. (D) Values are number of GFPpositive alimentary nuclei per animal +/- S.D. (n = 10-20) (*, p < 0.05).

Picture from Warnhoff K et al. (2014) PLoS Genet "The DAF-16 FOXO transcription factor regulates natc-1 to modulate stress ...."

Figure 3. NATC-1 protein is expressed in many cells and tissues and localizes to the cytoplasm. We generated transgenic natc-1(am138) animals expressing NATC-1::GFP fusion protein driven by the natc-1 promoter (WU1449). Confocal fluorescent microscope images of live animals are shown with head on the left and tail on the right. Green represents NATC-1::GFP fusion proteins, except for puncta in intestinal cells visible in panels A, C and D that reflect autofluorescent gut granules. (A) An image of an entire worm. NATC-1::GFP signal is visible in many cells and tissues throughout the animal, and the uniform staining pattern suggests cytoplasmic localization. (B-E) Higher magnification images display fluorescence in the pharynx (P.), sheath cells (SC.), intestinal cells (I*), distal tip cell (DTC.), vulva (V*) and body wall muscle (BWM.). Scale bar is 25 mm for all images.

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(K) Pgrl-8::GFP is expressed in two OLL neurons.

Picture from Huang CY et al. (2012) PLoS One "C. elegans EIF-3.K promotes programmed cell death through CED-3 ...."

Figure 2. EIF-3.K protein expression. (A) Western blot analysis of EIF-3.K protein expression. Affinity-purified anti-EIF-3.K antibodies were used to probe a blot of embryonic extracts from wild-type and eif-3.K(gk126) worms (above). Equal loading of the two extracts was confirmed by anti-a tubulin antibodies (below). The sizes of molecular weight markers and the positions of EIF-3.K and a tubulin are indicated. (B-D) Images of an eif-3.K(gk126)mutant early embryo (B), a wild-type early embryo (C) and a wild-type newly hatched larva (D) that were co-stained with anti-EIF-3.K antibodies and DAPI. Merged images are also shown. Scale bar = 10 um.

Picture from Manil-Segalen M et al. (2014) Dev Cell "The C. elegans LC3 acts downstream of GABARAP to degrade autophagosomes by ...."

(D-K) Electron micrographs of GFP::LGG-1 (D-H) or GFP::LGG-2 (J and K) embryos incubated with anti-LGG-1 (D and E) or anti-GFP (F-H, J, and K) antibodies revealing autophagosomes (D, F, and J), isolation membranes (E, G, and K) andoccasionally, the lumen of lysosomes (H). (D0-K0) Higher magnifications of the positive structures observed in (D-K). Scale bar represents 200 μm. (I) Quantification of the diameter of the autophagosomes and of the localization of gold beads are shown as mean ± SEM.

Picture from Li P et al. (2016) Neuron "Two Clathrin Adaptor Protein Complexes Instruct Axon-Dendrite ...."

(J-K) GFP-tagged SER-1 is co-expressed with a cytosolic tdTomato in AVE in wild type (J), apb-3(ok429)(K) animals. Arrows point to the dendritic region of the AVE neuron. Scale bar is 10um.

Picture from Gravato-Nobre MJ et al. (2016) PLoS Pathog "The Invertebrate Lysozyme Effector ILYS-3 Is Systemically Activated in ...."

(J-K) Representative images of the 4.5 Kb ilys-3 long promoter GFP construct in adult hermaphrodites. (J) Promoter activity in pharynx and intestine and (K) in the coelomocytes. ph:pharynx; int: intestine; cc: coelomocytes. Scale bars as indicated.

Picture from Cattie DJ et al. (2016) PLoS Genet "Mutations in Nonessential eIF3k and eIF3l Genes Confer Lifespan Extension and ...."

Fig 7. EIF-3.K::GFP is ubiquitously expressed in C. elegans and is localized to the cytosol. (A) Lifespan curves of wild-type, the eif-3.K mutant, and a strain containing a fluorescently-tagged allele of eif-3.K. (B) Developmental assay monitoring the growth and viability of the indicated genotypes on P. aeruginosa at 25 C. Error bars reflect the S.D. of 3 plates. (C) Fluorescent micrograph of EIF-3.K::GFP at 10x magnification. (D) Fluorescent confocal image depicting intestinal nuclei of L4 larvae of the indicated genotypes at 20x magnification. Arrows indicate intestinal nuclei.

Picture from Shen K et al. (2004) Cell "Synaptic specificity is generated by the synaptic guidepost protein SYG-2 and ...."

Images are taken from animals expressing SYG-1::GFP (kyIs288) (C, D, G, H, K, and L).(C and D) SYG-1 GFP and DIC image of a mid-L3 stage animal.(G and H). SYG-1 GFP and DIC image of a mid-L4 stage animal.(K and L). SYG-1 GFP and DIC image of a young adult. Arrows in (K) point to diffusely localized SYG-1 on HSNL axon outside of synaptic regions.