Picture from Ou G et al. (2007) Mol Biol Cell "Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components ...."

Schematics and fluorescence microscopy of C. elegans neuronal cilia. (J) Cilia structure schematics adapted from Ward et al. (1975). (N) Fluorescence microscopic images of neuronal cilia. (J and N) The 'fork' shaped AWB cilia, which are visualized using a GFP transcriptional fusion of a seven-span transmembrane receptor, STR-1 (N).

Picture from Ou G et al. (2007) Mol Biol Cell "Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components ...."

Schematics and fluorescence microscopy of C. elegans neuronal cilia. (D) Cilia structure schematics adapted from Ward et al. (1975). (H) Fluorescence microscopic images of neuronal cilia. (D and H) Outer-labial quadrant neuron (OLQ) cilia, and the GFP translational fusion of a TRPV channel protein OSM-9 marks their cylindrical shape.

Picture from Ou G et al. (2007) Mol Biol Cell "Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components ...."

Schematics and fluorescence microscopy of C. elegans neuronal cilia. (I) Cilia structure schematics adapted from Ward et al. (1975). (M) Fluorescence microscopic images of neuronal cilia. (I and M) The branched AWA cilia, which are marked by GFP translational fusions of a seven-span transmembrane odorant receptor, ODR-10 (M).

Picture from Ou G et al. (2007) Mol Biol Cell "Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components ...."

Schematics and fluorescence microscopy of C. elegans neuronal cilia. (L) Cilia structure schematics adapted from Ward et al. (1975). (P) Fluorescence microscopic images of neuronal cilia. (L and P) 'Finger'-shaped AFD cilia, which are marked by the distribution of GFP proteins driven by the promoter of a transmembrane guanylyl cyclase, GCY-8 (P). Bar, 5 um.

Picture from Kreutzer MA et al. (1995) J Cell Sci "Caenorhabditis elegans cyclin A- and B-type genes: a cyclin A multigene family, ...."

Fig. 5. The 1.3 kb cyclin B transcript appears specific to the paternal germline. In this northern blot, each lane contained 3 ug of poly(A)+ RNA. Lane 1, him-5 RNA; lane 2, staged L3/L4 RNA; lane 3, N2 mixed-stage (mostly adult) RNA. The probes used were: (A) the full-length C. elegans cyclin B cDNA; and (B) the full-length, 600 bp, C. elegansMSP cDNA (Burke and Ward, 1983). A pharyngeal myosin gene (Miller et al., 1986) was hybridized as a control to ensure equivalent loading of the RNA samples (not shown); for male RNA the vitellogenin probe used in Fig. 3 is not an appropriate control, as male worms do not produce vitellogenin (Schedin et al., 1991).

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 3. itx-1 expression. (A): itx-1prom::gfp expression in non-neuronal cells. (a) Intestinal expression in embryo and (b) in an L1 larva. (c) expression in spermatheca (white arrows) and (d) in vulva (white arrows). Blue star in (c) and (d) indicates gut expression. (B): itx-1prom::gfp expression in glial cells. (a) Expression in amphid socket cells; gfp signal is inverted for contrast; (b-e) co-labeling of itx-1-expressing sheath cells and sensory neurons filled with dye, as previously described (Perkins et al., 1986) (b), socket cells with itx-1prom::gfp (c), Normarksi image (d) and overlap of red and green in (e); (f): Sensory anatomy with associated sheath cell nuclei labeled in blue, socket cell nuclei in green and sensory neuron nuclei in red. Adapted from (Ward et al., 1975).

Picture from Ou G et al. (2007) Mol Biol Cell "Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components ...."

Figure 1. Schematics and fluorescence microscopy of C. elegans neuronal cilia. (A, C, D, I, and L) Cilia structure schematics adapted from Ward et al. (1975). (B) The schematic drawing of axonemal MTs in amphid channel cilia which contain 1-um-long transitional zone, 4-um-long middle segment with doublet MTs, and 2.5-um-long distal segment with singlet MTs. (E, G, H, M, and P) Fluorescence microscopic images of neuronal cilia. Amphid channel cilia are ASE, ADF, ASG, ASH, ASI, ASJ, ASK, and ADL (from left to right in A); among them ADF and ADL are biciliated, and they are visualized using the distribution of an IFT-particle protein OSM-6::GFP labeled with GFP (E). Phasmid channel cilia (C) can be labeled with GFP::IFT-particle proteins such as OSM-1::GFP (F) or OSM-6::GFP (G). PHA and PHB cilia individually extend their middle segments from a transition zone, and their distal segments enter together into a phasmid pore. Intraflagellar transport in phasmid cilia displays biphasic rates (F): kymographs with corresponding schematics showing the lines representing selected OSM-1::GFP particle trajectories along the middle segments (M and M') and the distal segments (D and D'), and motility along the distal segments is faster than along middle segments; horizontal bar, 2.5 um and vertical bar, 5 s in F. (D and H) Outer-labial quadrant neuron (OLQ) cilia, and the GFP translational fusion of a TRPV channel protein OSM-9 marks their cylindrical shape. (I and M) The branched AWA cilia, which are marked by GFP translational fusions of a seven-span transmembrane odorant receptor, ODR-10 (M). (J and N) The 'fork' shaped AWB cilia, which are visualized using a GFP transcriptional fusion of a seven-span transmembrane receptor, STR-1 (N). (K and O) The 'fan'-shaped AWC cilia, which can be labeled with the transcriptional fusion of the seven-span transmembrane receptor, STR-2 (O). (L and P) 'Finger'-shaped AFD cilia, which are marked by the distribution of GFP proteins driven by the promoter of a transmembrane guanylyl cyclase, GCY-8 (P). Bar, 5 um.