Picture from Wang J et al. (2010) Genetics "Functional specialization of sensory cilia by an RFX transcription factor ...."

Figure 4. daf-19m is exclusively expressed in male-specific PKD neurons and core IL2 neurons. (A) Discrete cis-regulatory elements regulate daf-19m expression in head (CEM/IL2) and tail (HOB/RnB) neurons. The CEM/IL2 and HOB/RnB elements are conserved among Caenorhabditis species C. elegans (Ce), C. briggsae (Cb), and C. remanei (Cr). (B) Diagram of daf-19m promoter::GFP reporters and their relationship to daf-19 and the n4132 deletion. (C and F) In hermaphrodites, P4daf-19m::GFP is expressed in IL2 neurons (C) and abolished in P5daf-19m::GFP by deleting the HOB/RnB element (F). (D and E, G and H) In the male, P4daf-19m::GFP is expressed in IL2 and CEM head neurons (D) and HOB and RnB tail neurons (E), and is completely abolished in P5daf-19m::GFP, which removes the 13-bp HOB/RnB element (G and H). Bar, 10 um.

Picture from Wang J et al. (2002) Development "The expression of TGFbeta signal transducers in the hypodermis regulates body ...."

Figure 2. The sma-3::gfp(C) expression stage and pattern in wild type. (A,C,D,F,H) Direct fluorescence from GFP; (B,E,G,I) Nomarski images of the same samples to their left. (A,B) The expression begins at the late embryo stage, 2.5-fold stage. (C) The gene expression pattern in L3 stage worms. At L4 stage, sma-3 is expressed in pharynx, head region hypodermis (D,E), intestine (F,G) and body hypodermis (H,I). The arrows indicate the hypodermal nuclei. The arrowhead indicates an intestinal nucleus.

Picture from Wang J et al. (2005) Development "C-terminal mutants of C. elegans Smads reveal tissue-specific requirements for ...."

Figure 1. Expression patterns of Smad constructs in vivo. (A) Localization of SMA-3 mutant forms. The SMA-3 AMA protein accumulates in the nucleus, similar to the accumulation of wild-type SMA-3. The other dominant-negative SMA-3 mutants have a similar localization pattern (data not shown). (B) The expression pattern of a sma-2::gfp transcriptional fusion gene in (B1) the whole animal, (B2) pharynx, (B3) intestine and (B4) hypodermis. (C) A functional genomic clone of sma-2 excluding the two large introns.

Picture from Wang J et al. (2005) Development "C-terminal mutants of C. elegans Smads reveal tissue-specific requirements for ...."

Expression patterns of Smad constructs in vivo. (A) Localization of SMA-3 mutant forms. The SMA-3 AMA protein accumulates in the nucleus, similar to the accumulation of wild-type SMA-3. The other dominant-negative SMA-3 mutants have a similar localization pattern (data not shown). (B) The expression pattern of a sma-2::gfp transcriptional fusion gene in (B1) the whole animal, (B2) pharynx, (B3) intestine and (B4) hypodermis. (C) A functional genomic clone of sma-2 excluding the two large introns.

Picture from Wang JC et al. (2004) J Biol Chem "The Caenorhabditis elegans ortholog of TRAP240, CeTRAP240/let-19, ...."

Figure 2. A, expression patterns of CeTRAP240-let-19. A reporter construct containing a 2.0-kilobase fragment encompassing the CeTRAP240-let-19 promoter and regulatory region-driving expression of GFP was injected into N2 hermaphrodites. Expression was monitored in different stages, tissues and cells: a, 200-cell stage embryo; b, vulva (arrow); c, neurons in tail, hypodermal cell (arrow), and skeletal muscle (yellow arrow); d, H-shaped excretory canal (arrow). B, CeTRAP240-let-19(RNAi) phenotypes. Depending on dosage of dsRNA, disruption of CeTRAP240-let-19 function by RNAi resulted in substantial embryonic lethality; survivors developed several phenotypes: a, embryonic arrest at 200-300 cell stage; b, abnormal head morphology; c, abnormal tail morphology; d, withered tail and cuticle shedding defect; e, multivulva. C, the C terminus of CeTRAP240/LET-19 is an intrinsic transcriptional repressor. A549 cells were transfected with various amounts of expression plasmids, as indicated, that encode either GAL4 DNA binding domain, GAL4-LET-19-C, or GAL4-TLE1 together with a (GAL4)5E1BLuc reporter (150 ng). The RSV-LacZ plasmid was also transfected to normalize transfection efficiency. Results shown here are representative of three independent experiments.

Picture from Wang JC et al. (2004) J Biol Chem "The Caenorhabditis elegans ortholog of TRAP240, CeTRAP240/let-19, ...."

A, expression patterns of CeTRAP240-let-19. A reporter construct containing a 2.0-kilobase fragment encompassing the CeTRAP240-let-19 promoter and regulatory region-driving expression of GFP was injected into N2 hermaphrodites. Expression was monitored in different stages, tissues and cells: a, 200-cell stage embryo; b, vulva (arrow); c, neurons in tail, hypodermal cell (arrow), and skeletal muscle (yellow arrow); d, H-shaped excretory canal (arrow).

Picture from Wang D et al. (2013) J Gen Physiol "GCY-8, PDE-2, and NCS-1 are critical elements of the cGMP-dependent ...."

(C) Fluorescent micrographs of double transgenic lines coexpressing mCherry in AFD and GFP under the control of promoters for each pde gene. Top row, promAFD::mCherry in the AFD neuron; middle row, GFP reporters for pde-2; bottom row, merged image. Bar, 10 um.

Picture from Wang D et al. (2013) J Gen Physiol "GCY-8, PDE-2, and NCS-1 are critical elements of the cGMP-dependent ...."

(C) Fluorescent micrographs of double transgenic lines coexpressing mCherry in AFD and GFP under the control of promoters for each pde gene. Top row, promAFD::mCherry in the AFD neuron; middle row, GFP reporters for pde-2; bottom row, merged image. Bar, 10 um.

Picture from Wang D et al. (2013) J Gen Physiol "GCY-8, PDE-2, and NCS-1 are critical elements of the cGMP-dependent ...."

Figure 4. Thermotaxis depends on at least four pde genes, of which two are expressed in AFD. (A and B) Thermotaxis response profiles of pde mutants at Tc = 15C and Tc = 20C. Bars are mean ± SEM; the number of assays is indicated in parentheses. *, P < 0.05 compared with wild type. (C) Fluorescent micrographs of double transgenic lines coexpressing mCherry in AFD and GFP under the control of promoters for each pde gene. Top row, promAFD::mCherry in the AFD neuron; middle row, GFP reporters for the pde-1, pde-2, pde-3, or pde-5 genes; bottom row, merged image. Bar, 10 um.

Picture from Wang Z et al. (2014) J Cell Biol "UNC-6 (netrin) stabilizes oscillatory clustering of the UNC-40 (DCC) receptor ...."

Figure 7. MADD-2 promotes UNC-40 clustering and polarization toward UNC-6. Anterior is left; ventral is down. (A) MADD-2::GFP was colocalized with F-actin (visualized with mCherry::moeABD) at the AC's invasive cell membrane in wild-type animals and in ectopic F-actin patches in unc-6 mutants, locations where UNC-40 resides (arrowheads; the location of basement membrane [BM] is indicated with broken lines; colocalization graphs on the right were measured along the yellow lines in the magnified insets below). (B) The dominant UNC-40-mediated F-actin patch polarized in random sections of the plasma membrane in the ACs of madd-2; unc-6 mutants (P > 0.1, 2 test, n = 24 animals observed; see Fig. 4 C). (C) F-actin cluster formation (arrowheads) was slower before disassembly in a madd-2; unc-6 mutant (shown over a 79-min time lapse). (D) The volume of F-actin patch formation (red line, dominant patch) in the madd-2; unc-6 animal shown in C. (E) Similar analysis of three additional madd-2; unc-6 mutants. (F) UNC-40::GFP and F-actin colocalized (arrowheads) in madd-2; unc-6 mutants (top) and in animals with RNAi-induced loss of madd-2 (bottom). UNC-40::GFP and F-actin were mispolarized after loss of madd-2 (arrowheads, bottom). (G) Quantification of UNC-40 polarity in wild-type and madd-2 (RNAi)-treated animals (n > 20 for each stage per genotype; ***, P < 0.001, Student's t test). Bars: (main panels) 5 um; (magnified insets) 1 um.