Figure 4. Thermotaxis depends on at least four pde genes, of which two are expressed in AFD. (A and B) Thermotaxis response profiles of pde mutants at Tc = 15C and Tc = 20C. Bars are mean ± SEM; the number of assays is indicated in parentheses. *, P < 0.05 compared with wild type. (C) Fluorescent micrographs of double transgenic lines coexpressing mCherry in AFD and GFP under the control of promoters for each pde gene. Top row, promAFD::mCherry in the AFD neuron; middle row, GFP reporters for the
pde-1,
pde-2,
pde-3, or
pde-5 genes; bottom row, merged image. Bar, 10 um.