Figure 3. PRG-1 Localizes to P Granules during Spermatogenesis. (A) Live animals expressing the PRG-1:GFP transgene were exposed to Hoechst dye and then gonads directly dissected and mounted for viewing. DIC and fluorescent images were collected. Scale bar: 10 μm.(B) The pachytene region of L4 and adult gonads from PRG-1::GFP transgenic animals are shown. Green, PRG-1::GFP; blue, Hoechst; scale bar: 10 μm.(C) PRG-1:GFP intensity was measured from equivalent exposures of pachytene germ cells from ten animals each at L4 and adult stages. Error bar represents the standard deviation. Asterisk, Student's t test, p < 0.01.(D) L4 hermaphrodite gonads were stained with anti-GFP (green) and anti-PGL-1 (red). Upper panels: 1000X magnification; scale bar, 5 μm; lower panels: 400X magnification.
Expression pattern of C01H6.9 (haspin; G) as determined by promoter-GFP reporter constructs. (G) C01H6.9 expression is shown in one of the phasmid neurons, PHB.
Expression of MBOA-7::GFP in an early embryo (D and E) and a second-stage larva (F and G). Nomarski micrographs (D and F) and corresponding MBOA-7::GFP expression (E and G). MBOA-7::GFP was ubiquitously expressed throughout development. Asterisks indicate the anterior of the larva (F and G). Bar, 10 um (D and E) and 200 um (F and G).
(B-C) An efn-2::GFP transcriptional reporter (Wang et al., 1999) shows GFP expression in hyp7 (arrowhead) during the time of DTC migration (B), but not before DTC migration (C). The bright spots in panel C are nonspecific gut autofluorescence.