Picture from Ji N et al. (2013) Cell "Feedback control of gene expression variability in the Caenorhabditis elegans ...."

Figure 1. Using Single-Cell Transcript Counting to Study the Control of mab-5 Expression (A) Schematic representation of the activation of MAB-5 expression in QL in response to the posterior- to-anterior gradient of EGL-20/Wnt. (B) Model of Wnt signaling based on published studies. Question marks and gray edges indicate lack of definitive evidence. (C) Final position of QL descendants in wild-type and various Frizzled loss-of-function mutants. Unless otherwise noted, compound mutants carry the same alleles as single mutants. (D) Detection of mab-5 transcripts using smFISH over the course of QL migration. Upper: QL at different stages of its migration. V5 is a stationary cell used as spatial reference. Lower: smFISH staining of mab-5 transcripts in the same cells as shown above. Yellow arrowheads: single mab-5 transcripts; white arrowheads: transcription centers in the nucleus. Scale bar represents 2.5 mm. (E) mab-5 transcription dynamics in single QL neuroblasts in wild-type animals. Upper: normalized total MD for worms collected at different time points after hatching. Black dots mark the mean, and blue bars span 2.5-97.5 percentiles. Lower: number of mab-5 transcripts per cell plotted against MD. The histogram to the right is generated using data points to the left with MD > 8. Black lines are generated by fitting to a sigmoidal function. Red curves are generated by fitting with two Gaussian distributions. (F) mCherry transcription dynamics in the POPTOP strain.

Picture from Yoshina S et al. (2015) PLoS One "Loss of C. elegans GON-1, an ADAMTS9 Homolog, Decreases Secretion Resulting in ...."

Fig 6. Depletion of gon-1 causes DAF-7 secretion defects. (a, b) Fluorescent images of DAF-7::mCherry, and DIC images (inset) in the head neurons of a wild-type animal (a) and a gon-1(tm3146) mutant animal (b) (160 msec. exposure time). The scale bar indicates 10 um. (c) Quantification of the mCherry fluorescence in neurons. The graph represents the combined results of 3 independent experiments (n > 20 neurons per strain). *p < 0.05. Bars represent themean SE. (d, e) Representative fluorescent images of DAF-7::mCherry in the coelomocytes of wild-type (d) and a gon-1(tm3146) mutant (e) animals (100 msec. exposure time). Coelomocytes are outlined with dotted circles in the fluorescent images. Insets show DIC images. The scale bar indicates 10 um. (f) Quantification of the mCherry fluorescence in coelomocytes. The graph represents the combined results of 3 independent experiments (n > 30 coelomocytesper strain). Fluorescence intensity was examined using ImageJ (NIH, Bethesda, MD). *p < 0.05. Bars represent the mean SE.IL is WBbt:0003888" Certain

Picture from Yoshina S et al. (2022) MicroPubl Biol "Integration of multicopy extrachromosomal transgenes into defined loci ...."

Figure 1. Isolation of multicopy integrant strains by the CRISPR/Cas9 system.(A) Procedures for isolation of multicopy integrant strains by the CRISPR/Cas9 system. Fluorescent markers are shown by green (EGFP) and red (DsRed). (B) Relative amounts of the lag-2 promoter and the T07F10.1 gene as determined by quantitative PCR. Primer set 1 is targeted at the lag-2 promoter. Primer set 2 is targeted at the T07F10.1 gene. The data were normalized with the ama-1 gene. The copy numbers are presented as a ratio to the wild-type N2 (n>3). (C) A scatter plot of the copy number of integrated sequences (abscissa) against fluorescence intensity (ordinate) in the Ex5672, and the 14 Is lines. The X axis is shown in log scale. The graph represents the intensity of fluorescence of DTC (y-axis, n > 20 animals per strain). Fluorescence intensity was measured using ImageJ (NIH, Bethesda, MD). (D) Example of GFP protein expressed in DTC. Scale bar = 5.0 μm. (2 sec. exposure time, gain X 4)