Picture from Roy, Vincent et al. MicroPubl Biol

Figure 1. mNG::PAR-4A & C are ubiquitously expressed, excluded from nuclei and enriched at the cell cortex. (A) Schematic depiction of par-4 locus organization and the predicted transcripts from this gene before editing (top), after homologous recombination (middle), and after SEC removal (bottom). Adapted from (Dickinson et al. 2015). (B-E) Fluorescence micrographs (B, D) and pseudo-DIC/fluorescence-merge micrographs (C, E) of wild-type (N2) animals and mNG::par-4a & c animals taken with the same settings, 24 hours (25 degrees C) after the late L4 stage. (F) Fluorescence micrograph showing a close-up of the germ line of a mNG::par-4a & c animal. (G) Fluorescence micrographs of 1- and 4-cell mNG::par-4a & c embryos. Scale bars: B-E, 80 mM; F, 55 mM; G, 27 mM. (H) Maternal effect embryonic lethality of the indicated genotypes at 25 degrees C.

Picture from Hulsey-Vincent H et al. (2023) MicroPubl Biol "A Fiji process for quantifying fluorescent puncta in linear cellular ...."

Figure 1. Identification and analysis of puncta using Fiji:A. The Fiji macro straightens a user-defined line-scan to identify fluorescent puncta based on parameters that can be used across datasets. B. An intensity profile of the punctum outlined in cyan. Puncta that are defined using the Fiji macro are analyzed so that data about intensity (X) and width (Full-Width at Half-Maximal intensity, (FWHM), dashed line) can be recorded and output as a CSV file. C. Example data from ventral nerve cords in animals expressing GLR-1::GFP under the control of the glr-1 promoter (nuIs24), N = 22. D. Comparison of puncta identification from a sample with distinct puncta (top two panels) and a sample with puncta that are less distinct from the nerve cord (bottom two panels). Images are from dorsal nerve cords in animals expressing GFP::SNB-1 under the control of the unc-129 promoter (nuIs152).

Picture from Hulsey-Vincent H et al. (2023) MicroPubl Biol "Comparison and agreement between two image analysis tools for quantifying ...."

Figure 1. Comparison of analyses of widefield and confocal images of GFP:SNB-1 using Igor and Fiji:A, B. Representative images of WT and fshr-1 mutant animals expressing GFP::SNB-1 imaged using widefield and confocal microscopy, respectively. C. Quantification of puncta intensity (peak-to-bead) per animal. Left, original quantification using IgorPro software; Center; analysis of the same data set using the Fiji macros (shown as bar graphs, with error bars representing SEM, left, and swarm plots, right); Right, quantification of images acquired using confocal microscopy by the Fiji macros displayed as swarm plots to show normalized intensity values for individual animals. Statistics for all plots are described in the text. D, E. Quantification of puncta width measured as Full-width Half-maximal (FWHM) fluorescence (D) and puncta per 10 𝜇m (E).

Picture from Hulsey-Vincent H et al. (2023) MicroPubl Biol "A Fiji protocol for analyzing puncta is a robust tool for measuring GLR-1::GFP ...."

Figure 1. Comparison of quantification of GLR-1:GFP puncta using Igor and Fiji software:A. Representative images of WT and daf-7 mutant animals expressing GLR-1::GFP under the control of the glr-1 promoter. B-D. Left, original quantification using IgorPro software; Center; analysis of the same data set using the Fiji macros. Error bars represent SEM. Right, ImageJ quantification displayed as swarm plots to show average intensity values for individual animals. B. Quantification of puncta intensity (peak-to-bead) per animal. C. Quantification of puncta width measured in microns as Full-width at Half-maximal (FWHM) fluorescence. D. Quantification of puncta density per 10 microns, by animal.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.