Figure 1. DYN-1 regulates microtubule dynamics in the PLM neuron. (A) Analyses of microtubule plus-end binding protein-2 (EBP-2) comets in WT (left panels) and
dyn-1(
ky51) (right panels) strains. Animals were incubated for at least 2 hours at 25°C before confocal imaging (top panels). Bars represent 10 μm. The corresponding kymographs (bottom panels) represent the movement of individual comets (white lines) over a distance of 14 - 15 μm (region of interest (ROI) shown in red). Horizontal and vertical bars represent approximately 2 μm and 20 seconds, respectively. (B) The number and (C) direction of EBP-2::GFP comets recorded in both WT and
dyn-1 animals pre-axotomy. (D) Analyses of individual EBP-2 comets measured 14-15 μm from the cut site (ROI shown in red) in WT and
dyn-1 animals 1-hour post-axotomy. The corresponding kymographs are shown below each image. Horizontal and vertical bars represent approximately 2 μm and 20 seconds, respectively. Animals were incubated at 25°C before and after axotomy. (E) The number and (F) direction of EBP-2::GFP comets recorded in both WT and
dyn-1 animals post-axotomy. P-values calculated using unpaired student t-tests; **p<0.005, n > 9 worms or more than 100 comets per genotype.