Figure 3. AAT-9 expression in the head region of wild type C. elegans. A, Nomarski image of the animal shown in panels B and C. B and C, the GFP fluorescence signal (green) is observed in neuronal bodies (arrows) and in body wall muscles (arrowheads) of
aat-9::gfp transgenic animals. The upper arrow in panel B indicates a neuron possibly corresponding to the one labeled by immunofluorescence (panel D). The signal in the posterior region (panel B, asterisks) is considered autofluorescence. In a different focal plane of the same worm (panel C), a specific GFP signal is seen in a symmetrical pair of presumably sensory neurons (upper arrow) localized ventral to the pharynx. D, immunofluorescence with AAT-9 antibody (red) shows a signal at the level of a neuronal body (arrow). Nuclei are stained with 4',6-damidino-2-phenylindole (blue). The punctuated signal in the anterior head region (asterisk) is considered nonspecific. Scale bars, 50 μm