Picture from Uebel, Celja J et al. MicroPubl Biol

Figure 1. PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. (A) Representative live images of endogenously tagged PGL-1::GFP (top row), PGL-1::mKate2 (middle row), and PGL-1::mTagBFP2 (bottom row) expression in the late pachytene and diplotene region of undissected C. elegans gonads. Scale bar, 10μm. (B) Live images of endogenously tagged PGL-1 in late pachytene zone of buffer dissected gonad. Scale bars, 5µm. (C) Live images of late pachytene region of gonads dissected in 5% 1,6-hexanediol, an aliphatic alcohol. Scale bars, 5μm. (D) Live images of undissected late pachytene region immediately after heat shock of 34C for 3 hours. Scale bars, 5μm. (E) Live images of pachytene region 5 hours after microinjection of 200μg/mL of the transcriptional inhibitor, +/--amanitin. Scale bars, 5μm.

Picture from Uebel, Celja J. et al. (2021) MicroPubl Biol

Figure 1. SIMR foci are numerous and bright in the Z2/Z3 progenitor germ cells: A-E: Representative live images of embryos expressing PGL-1::BFP (blue), MUT-16::GFP (green) and SIMR-1::mCherry (red) to visualize P granules, Mutator foci, and SIMR foci, respectively. Scale bars, 15 um. For each stage at least 3 embryos were observed. A'-E': Inset from boxed outline in A-E merge, highlighting the P lineage and progenitor germ cells. Scale bars, 1 um. C': Triangle arrowhead indicates early SIMR focus. D'-E': Notched arrowheads indicate bright, numerous SIMR foci (red) interacting with Mutator foci (green) and P granules (blue).