Figure 3. REPS-1 expression and function. (A) The
reps-1 locus. The deletion in the
tm2156 strain is also shown. (B) Protein lysates from wild-type and a transgenic line carrying a translational fusion of the
reps-1 gene with GFP (
reps-1p::REPS-1::GFP) were probed with indicated antibodies. (C) Images (epifluorescence) of hermaphrodites carrying the
reps-1p::REPS-1::GFP transgene. Anterior is to the left, ventral down. NR: nerve ring, INT: intestine, ESC: excretory system, V: vulva, NC: nerve cord, M: muscle cell. The asterisks indicate nerve commissures. Bars: 100 mm in a, 20 mm in b, 10 mm in c and d. (D) Aldicarb test on strains carrying mutations in EH proteins. Synchronized young adult animals were plated onto NGM plates containing 0.5 mM aldicarb and assayed after the indicated times for movement. The number of animals responding to a light touch with a platinum wire is reported. Results are the average of three independent experiments, each performed on 60 animals/strain. Note that the curves for N2 and
rme-1 worms overlap almost completely. P values are indicated in the table.