Picture from Trivedi M et al. (2023) MicroPubl Biol "CRISPR-mediated genome editing allows for efficient on demand creation of ...."

Figure 1. Methods and results on generating mini-deficiencies:A. Schematic showing the design for gRNA, ssDNA oligo repair, and genotyping primers for generating mini-deficiencies. H1 and H2 denote homology arms.B. Schematic showing the intended (black) and obtained (green) mini-deficiencies.C. Workflow for creating mini-deficiencies by injecting into balanced strains, and batch genotyping to detect successful edits. Agarose gel showing batch genotype result for dzDf1. Numbers on gel indicate lane/plate number.D. Workflow for creating mini-deficiencies by injecting into non-balanced strains, and a subsequent cross into balancer males to create a balanced strain.E. Agarose gel showing F1 single worm genotyping results of dzDf2 and dzDf3. Numbers above lanes indicate plate number with arrows marking lanes with PCR bands. Asterisks mark positive PCR results.F. Table summarizing the efficiency in generating the mini-deficiencies.G. Table summarizing efficiency of generating ChrI 2.0-2.1 Mb mini-deficiency under described conditions.H. Images of PVD neuron in wildtype, dz220 and dzDf1/dz220 background, showing non-complementation of dzDf1 with dz220.Created with BioRender.com (https://www.biorender.com/)

Picture from Goh KY et al. (2018) Biochim Biophys Acta "A large transcribed enhancer region regulates C. elegans bed-3 and the ...."

(A) bed-3 is expressed in the M lineage including M descendants and SM descendants.

Picture from Yang Q et al. (2017) Dev Cell "The Invading Anchor Cell Induces Lateral Membrane Constriction during Vulval ...."

(M-P) Localization of the endogenous GFP::TOCA-1 reporter zh110 in wild-type (M) and unc-6(ev400) mutants (O and P) before (M and O) and after (N and P)lumen formation. (M') and (O') show yz projections of the lateral VulF membranes. The inset in (O) shows the displaced AC in the unc-6(ev400) mutant in a differentfocal plane.

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).