Picture from Lu Q et al. (2011) Dev Cell "The WD40 repeat PtdIns(3)P-binding protein EPG-6 regulates progression of ...."

Q-T. Expression of gfp::epg-6 in the head region (Q), body wall muscles (R), the tail region (S) and the ventral cord (T) at larval stages.

Picture from Lin L et al. (2013) J Cell Biol "The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic ...."

At larval stages, W07G4.5::GFP forms aggregates in intestinal cells (Q and R).

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

Q and R, the nhx-9 promoter drove GFP expression in the excretory cell (ec) as well as in the phasmid sensory organs in the tail (phm) and the posterior intestine (int; AI and AJ).

Picture from Cordes S et al. (2006) Development "The C. elegans MELK ortholog PIG-1 regulates cell size asymmetry and daughter ...."

Figure 6. Expression and subcellular localization of PIG-1. (A-F) Images of larvae expressing Ppig-1::gfp: anterior, left; ventral, up. (A,C,E) DIC microscopy; (B,D,F) GFP fluorescence. (A,B) L1 larva expressing Ppig-1::gfp in the Q neuroblast (arrow) and V5 seam cell (arrowhead). (C,D) L1 larva expressing Ppig-1::gfp in the Q neuroblast daughters Q.a (arrow) and Q.p (arrowhead). (E,F) L1 larva expressing Ppig-1::gfp in the Q lineage descendants AQR (asterisk), AVM (arrow), and SQDR (arrowhead). (G,H) DIC (G) and fluorescence (H) images of an embryo expressing Ppig-1::pig-1::gfp. pig-1::gfp is present throughout the cytoplasm and excluded from the nucleus. Scale bars: 10μm.

Picture from Ono K et al. (2015) Mol Biol Cell "UNC-87 isoforms, Caenorhabditis elegans calponin-related proteins, interact ...."

(O-W) Expression ofGFP in hermaphrodite gonads with no transgene (O-Q), Punc-87A::GFP (R-T), or Punc-87B::GFP (U-W) was examined by immunostaining of dissected gonads for GFP (O, R, and U) and MYO-3 as a marker of myoepithelial sheath (P, S, andV). Merged images are shown in Q, T, and W (green, GFP; red, MYO-3; blue, DNA [not shown as individual images]). Scale bars, 100 um (whole worms; A, B, I, and J), 20 um (magnified tissues; C-H,K-N), 50 um (dissected gonads; O-W).

Picture from Stavoe AK et al. (2016) Dev Cell "KIF1A/UNC-104 Transports ATG-9 to Regulate Neurodevelopment and Autophagy at ...."

Figure 6. ATG-9 Transport to AIY PresynapticZones Requires KIF1A/UNC-104(A) Schematic of the nematode nerve ring (green, encircled by a dotted line) in C. elegans.(B) Schematic of the atg-9 genomic region in a wild-type genome (top) and in the enhanced GFP CRISPR knockin (bottom). (C and D) Location of the nerve ring, referenced with a transmitted light image (C) and visualized with GFP::RAB-3 (pseudocolored red) expressed panneuronally with Prab-3 in a wild-type animal (D).(E) Visualization of the subcellular localization of panneuronal ATG-9 (ATG-9::GFP rescuing array expressed with Punc-14). (F-K) Distribution of endogenous ATG-9 as visualized by CRISPR insertion of enhanced GFP at the C terminus of the genomic atg-9 (schematized in (B)) in adult (F-H) and embryo (I-K) in wild-type (G and J) and unc-104(e1265) mutant (H and K) animals. Anterior end of the embryo (head) is indicated with an asterisk in (I)-(K).(L-Q) Distribution of synaptic vesicles (visualized with mCh::RAB-3) (L and O) and ATG-9::GFP (M and P) in AIY (merge in N and Q) in wild-type(L-N) and unc-104(e1265) mutant animals (O-Q). In (L)-(Q), the asterisk denotes the location of the AIY cell body; the dashed box encloses AIYzone 2. Each image is a maximal projection of a confocal z stack. Scale bar in (D) for (C)-(H), in (J) for (I)-(K), and in (L) for (L)-(Q), 5 um.

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

Fluorescent profiles of transgenic nematodes expressing NHX-8::GFP translational fusion protein. A genomic fragment containing the promoter and entire open reading frame of nhx-8 gene was fused in-frame to GFP. The subcellular location of the fusion protein was determined by either conventional or confocal fluorescent microscopy. Fluorescent images are shown next to the corresponding differential interference contrast photomicrographs. Q and R, NHX-8::GFP is expressed in the seam cells and other tissues (see Results), and the NHX-8::GFP protein appears to accumulate in a punctate, perinuclear expression pattern (inset of Q).

Picture from Baum PD et al. (1997) Neuron "Neuronal migrations and axon fasciculation are disrupted in ina-1 integrin ...."

INA-1 Expression Pattern. (D and E) L1 larvae bearing the ina-1-GFP reporter construct gmIs5. Anterior is to the left, and ventral is down.(D) INA-1-GFP is expressed in the migrating Q cell lineage, beginning with the initial migrations of the Q cells. Here, the QR and QL daughters are shown. The photomicrograph shows the right side of the animal, with the left side of the same animal in the inset.(E) Neuronal expression of INA-1-GFP is seen in the head and ventral nerve cord (arrowheads). The scale bar is 10 μm.

Picture from Josephson MP et al. (2016) Genetics "The Caenorhabditis elegans NF2/Merlin Molecule NFM-1 Non-autonomously ...."

Figure 4 nfm-1 was not expressed in Q cells during their early migrations. (A-C) Ventral view of the posterior region of a Pnfm-1::gfp; Pegl-17::mCherrytransgenic animal staged to 3-3.5 hr posthatching. (A) GFP micrograph showing expression of Pnfm-1::gfp. Expression was seen in posterior cells nearthe anus, including posterior intestinal cells (Int) and the three rectal gland cells (Rect). Other unidentified cells in the region were possibly the anal sphincter muscle and the stomatointestinal muscle. Variable hypodermal expression was observed along the length of the animal (Hyp). (B) An mCherrymicrograph shows Q-cell-specific expression during their migrations. (C) Merged. GFP is not observed in Q cells, but is expressed in neighboring tissues and posterior cells. Bar, 10 um for A-C. (D-F) An L1 animal 3-3.5 hr posthatching with Pnfm-1::gfp expression in body wall muscles. Bar, 20 um forD-F.

Picture from Au C et al. (2009) PLoS One "SMF-1, SMF-2 and SMF-3 DMT1 orthologues regulate and are regulated ...."

An antero-posterior gradient of smf-2 expression is noticeable in the 9 marginal epithelial cells of the pharynx (mc1, mc2, mc3) and the 6 vpi cells of the pharyngeo-intestinal valve (N and O). Fainter expression is consistently observed in the proximal gonad (P, Q). Scale bars are 5 um.