Figure 6. ENPP-1::mScarlet is transferred from the male reproductive tract to the her- maphrodite uterus during copulation
x2028;(A) ENPP-1::mScarlet is present in the somatic part of the male reproductive tract in the vacuoles of cuboidal cells of the vas deferens. Zoomed inset shows a single plane through the giant vacuoles. Also see Video S2 for full z stack through the giant vacuoles showing appearance of speckled material with ENPP-1::mScarlet. (B) Male-to-hermaphrodite transfer experiment where ENPP-1::mScarlet males were mated to non- fluorescent hermaphrodites.
x2028;(C) Imaging of the non-fluorescent hermaphrodite af- ter copulation revealed presence of male-derived ENPP-1::mScarlet in the uterus. Yellow asterisks indicate intestinal autofluorescent droplets. See Video S3 for real-time imaging of male transferring ENPP-1::mScarlet into the hermaphrodite uterus. (D) Venn diagram showing overlap of the identified EV proteome with products of the male-enriched tran- scripts.47 (E) Scatter plot showing EV cargo predicted to be of either neuronal or glial origin. See Figure S6 for enrichment of identified EV cargo candidates in other tissues (hypodermis and germline) and for RNA profiling of PKD-2::GFP EV fractions.