Figure 1. Strain RB2126 contains background mutation(s) that affect the locomotion behavior of
flp-1(
ok2811) mutants: (A) Strain RB2126 (
flp-1(
ok2811);
daf-11(
yak155);
yak156) contains a background mutation in a constitutive dauer gene. Representative photos of dauers from the RB2126 strain (Upper panel) and the
daf-11(
sa195) mutant strain JT195 (Lower panel) grown at room temperature (23&#
x2DA;C). (B) Strain RB2126 has background mutation(s) that reduce the deep body bends of
flp-1(
ok2811) mutants. Photos of worm tracks from wild type (WT), XZ2314
flp-1(
ok2811), RB2126
flp-1(
ok2811);
daf-11(
yak155);
yak156, XZ2380
flp-1(
ok2811);
daf-11(
sa195), XZ2406
flp-1(
ok2811);
daf-11(
yak155), and XZ2394
flp-1(
ok2811);
yak156 strains. (C) Representative photos of young (~L3) XZ2314
flp-1(
ok2811), XZ2380
flp-1(
ok2811);
daf-11(
sa195), and XZ406
flp-1(
ok2811);
daf-11(
yak155) strains showing the characteristic "8"-like body posture. (D), (E), (F), (G) Quantification of the track waveform of the strains shown in Figure 1B (see Methods). The wavelength and amplitude of sinusoidal tracks in millimeters (mm) of strains N2 (WT), XZ2314
flp-1(
ok2811), RB2126
flp-1(
ok2811);
daf-11(
yak155);
yak156, and XZ2394
flp-1(
ok2811);
yak156 are also shown in figures (D) and (G). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. Error bars = SEM; n= 24-45.