Picture from Weeks JC et al. (2016) MicroPubl Biol "Microfluidic EPG Recordings Show Striking Pharyngeal Pumping Phenotype in a C. ...."

Reviewed by: Chris Link; Nicole Liachko.

Picture from Clark-Maquire SD et al. (1994) J Cell Biol "Localization of the mei-1 gene product of Caenorhaditis elegans, a ...."

Figure 4. MEI-1 staining in wild-type males and postmeiotic embryos. (A-C) Meiosis in the germline of males. Arrows in A show poles of two meiotic figures stained with anti-tubulin. No anti-MEI-1 staining is visible in B. DAPI staining in C shows that the chromosomes in the cell in the upper left are at the metaphase plate (arrow, chromosomes are seen as one brightly staining mass). The chromosomes in the cell at the lower fight are at the poles (arrows). Note the chromosome lagging at the metaphase plate in the latter cell; this is probably the unpaired X chromosome in the X0 male (see Albertson and Thomson, 1993). (D and E) Two cell embryo entering the third round of cleavage. Spindles are seen forming in D with anti-tubulin staining while no anti-MEI-1 staining is visible in this embryo (E). (F and G) Staining of the sperm nucleus. DAPI and anti- MEI-1 staining, indicated by the large arrows in F and G,respectively, shows that the embryo is in telophase of meiosis I (compare to Fig. 3, H and I). The sperm nucleus (small arrows)is stained in both panels. Note other regions stained by DAPI in F are not visible in G, indicating that the anti-MEI-1 staining of the sperm nucleus is not spill-over from the DAPI channel. Anterior is to the left in (D-G). Bars: (A) 3 um; (F) 5 um.