Picture from Krause MW et al. (1997) Development "A C. elegans E/Daughterless bHLH protein marks neuronal but not striated muscle ...."

Figure 3. CeE/DA antibody-positive pharyngeal nuclei. (A) Exploded view of the pharynx showing the location of muscle cell groups (pm1-8) (from Albertson and Thompson, 1975). (B) Abbreviated cell lineage diagram showing the origin of posterior pharyngeal nuclei relative to the founder blastomeres (AB, MS, E, C, D and P4). CeE/DA-positive nuclei are indicated by bold type; smaller, normal type indicates similar nuclei that are undetectable with the antibody. For pm5, CeE/DA-positive nuclei are AB descendants and negative nuclei are MS descendants. For g1 and g2 nuclei, no clear lineage distinction exists for CeE/DA-positive versus -negative nuclei.

Picture from Olahova M et al. (2008) Proc Natl Acad Sci U S A "A redox-sensitive peroxiredoxin that is important for longevity has tissue- and ...."

Fig. S7. PRDX-2 is widely expressed during embryogenesis and in multiple tissues postembryonically. Immunostaining with anti-PRDX-2 antibodies of wild-type (N2) embryos (A), an extruded gonad dissected from an adult hermaphrodite (B), and larvae (C and D) revealed that PRDX-2 is expressed during embryogenesis (A) and postembryonically in the gonad (the mitotic region is indicated) (B), neurons (C), and intestine (D). Comparisons with DAPI-stained DNA (A and D) suggest that PRDX-2 is excluded from the nucleus. Consistent with its activity as a thioredoxin peroxidase, PRDX-2-immunostaining produced a punctate staining pattern in intestinal cells similar to the localization of thioredoxin [Jee C, Vanoaica L, Lee J, Park BJ, Ahnn J (2005) Thioredoxin is related to life span regulation and oxidative stress response in Caenorhabditis elegans. Genes Cells 10:1203-1210]. Differential interference contrast light images (DIC) are also shown.

Picture from Tobin DM et al. (2002) Neuron "Combinatorial expression of TRPV channel proteins defines their sensory ...."

Figure 1. Sequence Analysis and Expression Pattern of the ocr Gene Family. (A) Alignment of the four predicted C. elegans OCR proteins. Amino acid sequences are based on sequencing of RT-PCR-generated cDNAs (ocr-1 and ocr-2) or on refinement of Genefinder predictions (ocr-3 and ocr-4). Identical residues are shaded black; similar residues are shaded gray. Solid lines denote predicted transmembrane domains, and dashed lines denote ankyrin repeats. OSM-9 is 937 amino acids long; its extreme C terminus is not shown.(B) Dendrogram of the TRPV subfamily of TRP-related ion channels. Sequences were aligned using the ClustalW alignment program (Thompson et al., 1994), and the dendrogram was constructed using Phylip software. Mammalian TRPV5 and TRPV6 (ECaC) fall on the same branch as the other mammalian TRPV channels.(C-G) Expression patterns of the ocr genes. Translational GFP fusions were constructed for each of the four ocr genes; each fusion includes the first 68-100 amino acids of the OCR protein. (D) OCR-1::GFP is expressed in the amphid sensory neurons AWA and ADL, indicated by arrows. The animal shown is mosaic, so only the right ADL neuron (ADLR) and left AWA neuron (AWAL) are visible. (E) OCR-2::GFP is expressed in AWA, ADL, ASH, indicated by arrows, as well as ADF and the phasmid neurons PHA and PHB (data not shown). With this transgene, AWA expression is weak compared to ASH and ADL. (F) OCR-3::GFP is expressed in the rectal gland cells, indicated by arrows. (G) OCR-4::GFP is expressed in the OLQ neurons; the OLQ cell bodies are indicated by arrows. The intersection of these expression patterns with that of osm-9 is represented in the Venn diagram in (C). Scale bar, 30 μm.

Picture from Fukuto HS et al. (2004) Neuron "G protein-coupled receptor kinase function is essential for chemosensation in ...."

Figure 2. Ce-grk-2 Encodes a G Protein-Coupled Receptor Kinase. (A) Predicted domain structures of Ce-GRK-2, human GRK2 (hGRK2), and human GRK3 (hGRK3) proteins. Ce-GRK-2 is 66% and 65% identical overall to hGRK3 and hGRK2, respectively. RGS, regulator of G protein signaling protein homology domain; PH, pleckstrin homology domain. The amino acid identity (%) between each human GRK and Ce-GRK-2 domain is indicated. The rt97 mutation corresponds to a T354I change in the predicted kinase domain of Ce-GRK-2.(B) Threonine 354 is conserved among serine/threonine kinases. Predicted kinase domains were aligned using the ClustalW program (Thompson et al., 1994) in the MegAlign software package (DNASTAR). The threonine residue equivalent to T354 of Ce-GRK-2 is indicated in the gray rectangle. The signature DLG motif of GRKs (top three sequences) and the DFG motif of other kinases (bottom three sequences) are indicated by the brackets. h, human; Sc, S. ceravisiae.(C and D) A Ce-grk-2::gfp transcriptional reporter is expressed in neurons in the body (C) and head (D). Broad expression in the nervous system and the vulval muscles is observed. Scale bar equals 100 μm in (C) and 25 μm in (D).(E and F) Ce-GRK-2 overexpressed using its own promoter can be detected in the nervous system with anti-mammalian GRK2/3 antibody. The entire animal (E) or just the head of the animal (F) are shown. Scale bar equals 100 μm in (E) and 25 μm in (F). No overt localization to sensory neuron cilia was observed, although overexpression of Ce-GRK-2 may mask or interfere with normal subcellular localization.