Picture from Thompson BE et al. (2005) Development "Dose-dependent control of proliferation and sperm specification by ...."

(A-B) Animals or dissected germlines stained with anti-FOG-1 antibodies. Broken lines indicate germlines in A-D. (A) Wild-type L2. FOG-1 is barely detectable. (B) Wild-type L3. FOG-1 is abundant in proximal germline where cells are destined to become sperm; FOG-1 is low in distal germline where cells remain in mitotic cell cycle. Scale bars: 10 um.

Picture from Thompson BE et al. (2005) Development "Dose-dependent control of proliferation and sperm specification by ...."

Figure 6. FBF represses fog-1 expression in vivo. (A-J) Arrow indicates distal end. Scale bars: 10 um. (A-E,G-J) Animals or dissected germlines stained with anti-FOG-1 antibodies. Broken lines indicate germlines in A-D. (A) Wild-type L2. FOG-1 is barely detectable. (B) Wild-type L3. FOG-1 is abundant in proximal germline where cells are destined to become sperm; FOG-1 is low in distal germline where cells remain in mitotic cell cycle. (C,D) L2 fbf-1 fbf-2. FOG-1 is more abundant than in wild type (compare with A) and its distribution is not graded. (D) L3 fbf-1 fbf-2. FOG-1 is more abundant than in wild type (compare with B) and its distribution is not graded. (E) Wild-type adult male germline. MR, mitotic region; TZ, transition zone. FOG-1 levels are low in mitotic region and become high as germ cells begin entry into meiosis in proximal part of mitotic region and transition zone. FOG-1 disappears in more mature stages of meiotic prophase. (F) Wild-type adult male germline hybridized with fog-1 antisense probe. fog-1 mRNA has same pattern as FOG-1 protein (compare with E). Sense probe has no detectable staining (not shown). (G) fog-1(q250) homozygous male germline. No FOG-1 protein is detectable. (H) Wild-type adult hermaphrodite germline. No FOG-1 protein is detectable. (I) Adult gld-3 nos-3 tumorous germline. FOG-1 levels are low. (J) Adult fbf-1 fbf-2 gld-3 nos-3 tumorous germline. FOG-1 levels increase in the absence of FBF (compare with I).

Picture from Thompson FJ et al. (2002) Biochem J "Biochemical and molecular characterization of two cytidine deaminases in the ...."

Figure 5. The Ce-cdd-1 reporter construct is expressed predominantly in the early larval stages. N2 worms were transfected with the Ce-cdd-1 translational fusion reporter gene construct, and stained for LacZ expression for 10 min. Worms were viewed on an Olympus BX60 fluorescence microscope. (A) Bagged adults containing eggs and hatched L1 (magnification i14); (B) L3 (magnification i28); and (C) L4 and adult (magnification i14). Especially noteworthy is staining of the intestinal cells, and intense staining of the pharyngeal bulbs.

Picture from Thompson-Peer KL et al. (2012) Neuron "HBL-1 patterns synaptic remodeling in C. elegans."

(A and B) A representative image and summary data of hbl-1 (HgfpH, green) and GAD (red) reporter expression in wild-type L3 animals. Yellow arrows indicate DD cell bodies expressing both markers, white arrows indicate DD cell bodies lacking HgfpH expression, and carrots indicate VD cell bodies. HgfpH expression was significantly lower in VD than DD neurons (p < 105 by Kolmogorov-Smirnov test; 150 DD (black) and 260 VD (gray) cells were analyzed).

Picture from Thompson FJ et al. (2002) Biochem J "Biochemical and molecular characterization of two cytidine deaminases in the ...."

Figure 6. The Ce-cdd-2 reporter construct is expressed throughout the life cycle N2 worms were transfected with the Ce-cdd-2 translational fusion reporter gene construct and stained for LacZ expression for 4.5 h. Worms were viewed on an Olympus BX60 fluorescencemicroscope. (A) Egg, i20; (B) L2, i20; (C) L4, i10; (D) adult, i10. Note staining is confined to the intestinal cells.

Picture from Thompson-Peer KL et al. (2012) Neuron "HBL-1 patterns synaptic remodeling in C. elegans."

Figure 2. UNC-55 COUP-TF Represses hbl-1 Expression in VD Neurons (A and B) A representative image and summary data of hbl-1 (HgfpH, green) and GAD (red) reporter expression in wild-type L3 animals. Yellow arrows indicate DD cell bodies expressing both markers, white arrows indicate DD cell bodies lacking HgfpH expression, and carrots indicate VD cell bodies. HgfpH expression was significantly lower in VD than DD neurons (p < 105 by Kolmogorov-Smirnov test; 150 DD (black) and 260 VD (gray) cells were analyzed). (C-F) HgfpC (C and D) or HmutgfpC (E) transcriptional reporter expression (green) is compared for adjacent VD10 and DD5 neurons in wild-type (C and E) and unc-55 (D) mutant animals. Gray lines connect VD10 and DD5 cells in the same animal, black lines connect median values (pvalues by paired Student's t test). Average log2 of the ratio of DD5 to VD10 fluorescence for HgfpC and HmutgfpC was plotted in (F), and n = number of animals analyzed (*p < 105 difference from WT; ns, p = 0.2; by Student's t test). Error bars indicate SEM. See also Figure S2.

Picture from Thompson HM et al. (2002) Curr Biol "The large GTPase dynamin associates with the spindle midzone and is required for ...."

Figure 3. Dynamin Localizes to the Newly Formed Furrow Membranes and the Midbody in C. elegans. All embryos are arranged with anterior toward the left.(A-E) Cells at the middle and late stages of mitosis were stained for alpha-Tubulin (green) and Dyn-1 (red) and were observed with a Leica confocal microscope as described previously [36]. ([A], arrow) At the metaphase-anaphase transition, dynamin was observed to localize between the asters. (B) During anaphase, dynamin appeared to localize equatorially, and a population of dynamin appeared on the midzone microtubules. ([C], arrow) During late telophase, dynamin appeared to localize only to the newly formed membrane. ([D], arrow) During early cytokinesis, dynamin localized to the newly formed membrane and along the cleavage furrow. ([E], arrow) As cytokinesis progressed, the dynamin staining became more punctate along the newly ingressed furrow, and the midbody staining was more intense.(F-I') To deplete C. elegans embryos and adults of dynamin, embryos and adults were treated with dyn-1 RNAi and were then stained, as described previously [36], for (F-I) microtubules (as a marker for the stages of mitosis) and (F'-I') dynamin. (G' and I') A marked reduction in dynamin staining as well as a (G and I) disruption of the microtubule cytoskeleton can be seen in the (G and G') early embryo and (I and I') adult gonad treated with dyn-1 RNAi as compared to (F and F' and H and H'; all gonad images are of the same focal plane) untreated control animals.(J and J') In the wild-type C. elegans gonad, dynamin resides just inside the tubulin meshwork. (J) Top and (J') midfocal planes of merged alpha-Tubulin (green) and dynamin (red) staining in the gonad are shown.The scale bars represent 5 μm.

Picture from Thompson BE et al. (2006) Proc Natl Acad Sci U S A "Germ-line induction of the Caenorhabditis elegans vulva."

FBF-1 is observed in germ line but not in VPCs. Late L2s. MH27 (green) outlines VPCs. Arrowheads indicate VPCs and dashed lines outline the germ line. MH27 recognizes cell adherens junctions (1) and outlines the VPCs (2). FBF-1 is readily detectable in germ cells but not in VPCs.

Picture from Thompson BE et al. (2006) Proc Natl Acad Sci U S A "Germ-line induction of the Caenorhabditis elegans vulva."

FOG-1 is observed in germ line but not in VPCs. Late L2s. MH27 (green) outlines VPCs. Arrowheads indicate VPCs, Insets show positive germ-line staining, and dashed lines outline the germ line. MH27 recognizes cell adherens junctions (1) and outlines the VPCs (2). FOG-1 is faint, but detectable, in germ cells but not in VPCs.

Picture from Thompson BE et al. (2006) Proc Natl Acad Sci U S A "Germ-line induction of the Caenorhabditis elegans vulva."

FBF-2 is observed in germ line but not in VPCs. Late L2s. MH27 (green) outlines VPCs. Arrowheads indicate VPCs, Insets show positive germ-line staining, and dashed lines outline the germ line. MH27 recognizes cell adherens junctions (1) and outlines the VPCs (2). FBF-2 is detectable in germ cells but not in VPCs.