Figure 2. Expression of
zag-1.
zag-1::YFP: DIC (A-C) and corresponding epifluorescence images (D-F) of embryos at different developmental stages.
zag-1 expression is mainly seen in neurons in the head and tail ganglia as well as in ventral cord motorneurons. Stars (F) mark two pharyngeal nuclei, probably
m4 and
m5 muscle cell nuclei. (G-H) Early (G) and late (H) L1 larvae; expression is seen transiently in postembryonic motorneurons (H). Arrowheads (H) indicate motorneuron nuclei in the ventral cord. Pzag-1::YFP in wildtype (I) and
zag-1(
rh315) animals (J-L);
zag-1 expression in neurons is not downregulated in
zag-1(
rh315). Arrows (I,J,L) indicate intestinal muscle, arrowheads (I,J,L) indicate the anal depressor muscle. Ventral aspects in (I,J), side views in all others. Anterior is to the left, dorsal is up. Scale bars: 20 um.